anti-O-GlcNAc antibody [9H6]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [9H6] recognizes O-GlcNAc
  • 反应物种: Other
  • 应用: ICC/IF, WB
  • 特异性: Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 9H6
  • 同位型: IgM
  • 靶点名称: O-GlcNAc
  • Brand:
CAT.NO. : ARG22637
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Product Details
概述
产品描述Mouse Monoclonal antibody [9H6] recognizes O-GlcNAc
反应物种Other
应用ICC/IF, WB
特异性Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
宿主Mouse
克隆Monoclonal
克隆号9H6
同位型IgM
靶点名称O-GlcNAc
抗原物种Others
抗原Synthetic β-O-Linked N-Acetylglucosamine conjugated to KLH.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50
WB1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化PEG Purified.
缓冲液PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol.
抗菌剂0.9% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (2)
  • ARG22637 anti-O-GlcNAc antibody [9H6] ICC/IF image

    Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22637 anti-O-GlcNAc antibody [9H6] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Primary antibody (D) Composite. .

  • ARG22637 anti-O-GlcNAc antibody [9H6] WB image

    Western blot: 2.0 µg of BSA, GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, Glucose-BSA, and Citrulline-BSA stained with ARG22637 anti-O-GlcNAc antibody [9H6] at 1:1000 for 2 hours at RT.

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