anti-OAT antibody [23A2]
产品描述 | Mouse Monoclonal antibody [23A2] recognizes OAT |
---|---|
反应物种 | Hu, Ms |
应用 | FACS, ICC/IF, WB |
宿主 | Mouse |
克隆 | Monoclonal |
克隆号 | 23A2 |
同位型 | IgG1, kappa |
靶点名称 | OAT |
抗原物种 | Human |
抗原 | Recombinant fragment around aa. 33-439 of Human OAT. |
偶联标记 | Un-conjugated |
別名 | OATASE; OKT; Ornithine aminotransferase, mitochondrial; Ornithine--oxo-acid aminotransferase; GACR; HOGA; Ornithine delta-aminotransferase; EC 2.6.1.13 |
应用建议 |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
---|---|
纯化 | Purification with Protein G. |
缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 10% Glycerol |
浓度 | 1 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # P04181 Human Ornithine aminotransferase, mitochondrial Swiss-port # P29758 Mouse Ornithine aminotransferase, mitochondrial |
---|---|
基因名称 | OAT |
全名 | ornithine aminotransferase |
背景介绍 | This gene encodes the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA. Mutations that result in a deficiency of this enzyme cause the autosomal recessive eye disease Gyrate Atrophy. Alternatively spliced transcript variants encoding different isoforms have been described. Related pseudogenes have been defined on the X chromosome. [provided by RefSeq, Jan 2010] |
预测分子量 | 49 kDa |
ARG56993 anti-OAT antibody [23A2] ICC/IF image
Immunoflorescense: HeLa cells stained with ARG56993 anti-OAT antibody [23A2] 1:500 (Red).
Hoechst 33342 (Blue) for nucleus staining.
ARG56993 anti-OAT antibody [23A2] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56993 anti-OAT antibody [23A2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).
ARG56993 anti-OAT antibody [23A2] WB image
Western blot: 35 µg of Mouse liver stained with ARG56993 anti-OAT antibody [23A2] at 1:1000.
ARG56993 anti-OAT antibody [23A2] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG56993 anti-OAT antibody [23A2] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56993 anti-OAT antibody [23A2] WB image
Western blot: 1) 20 ng of OAT recombinant protein, 2) 40 ug of 293T cell lysate, and 3) 40 ug of Brain tissue lysate stained with ARG56993 anti-OAT antibody [23A2] at 1:1000.
