anti-OCT4 antibody [SQab20238]
![anti-OCT4 antibody [SQab20238]](/upload/image/products/ARG66826_IHC-P_1_210_205.jpg)
![anti-OCT4 antibody [SQab20238]](/upload/image/products/ARG66826_IHC-P_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG66826
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Product Details
概述
| 产品描述 | Recombinant Rabbit Monoclonal antibody [SQab20238] recognizes OCT4 |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 克隆号 | SQab20238 |
| 同位型 | IgG |
| 靶点名称 | OCT4 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human OCT4. |
| 偶联标记 | Un-conjugated |
| 別名 | OTF-3; OCT4; OCT3; Octamer-binding protein 4; OTF3; Oct-3; Oct-4; OTF4; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Octamer-binding protein 3 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.01% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q01860 Human POU domain, class 5, transcription factor 1 |
|---|---|
| 基因名称 | POU5F1 |
| 全名 | POU class 5 homeobox 1 |
| 背景介绍 | This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013] |
| 生物功能 | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Note=Expressed in a diffuse and slightly punctuate pattern. [UniProt] |
| 预测分子量 | 39 kDa |
| 翻译后修饰 | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity). Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. [UniProt] |
检测图片 (1)
ARG66826 anti-OCT4 antibody [SQab20238] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human seminoma tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66826 anti-OCT4 antibody [SQab20238].
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