anti-PAK1 / PAK2 / PAK3 phospho (Thr402) antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
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- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG52387
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PAK1 / PAK2 / PAK3 phospho (Thr402) |
|---|---|
| 反应物种 | Rat |
| 预测物种 | Hu, Ms, Bov, Dog |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PAK1 / PAK2 / PAK3 |
| 抗原物种 | Rat |
| 抗原 | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | PAKalpha; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p65-PAK; EC 2.7.11.1; PAK-1; p21-activated kinase 1 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for the ~68k to ~70k PAK protein phosphorylated at Thr402. The immunolabeling of PAK is completely eliminated by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P35465 Rat Serine/threonine-protein kinase PAK 1 |
|---|---|
| 基因名称 | PAK1-3 |
| 全名 | p21 protein (Cdc42/Rac)-activated kinase 1 |
| 背景介绍 | In mammals, there are several identified isoforms of p21-activated protein k inases or PAKs: α-PAK (also known as PAK-1) and β-PAK (also known as PAK-3) are mostly brainspecific, while -PAK (also known as PAK-2) is expressed ubiquitously (Jakobi et al., 2003). Mutations of the gene coding for PAK-3 are associated with X-linked mental retardation and recent work indicates that PAK-3 is a key regulator of synapse formation and plasticity in the hippocampus (Boda et al., 2004). PAK-3 is thought to play a key role in regulation of cell shape and motility as well as cell death (Jakobi et al., 2003; Walter et al., 1998). Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK (Jakobi et al., 2000). |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites. |
检测图片 (1)
ARG52387 anti-PAK1 / PAK2 / PAK3 phospho (Thr402) antibody WB image
Western blot: Rat hippocampal lysate stained with ARG52387 anti-PAK1 / PAK2 / PAK3 phospho (Thr402) antibody showing specific immunolabeling of the ~68 kDa to ~70 kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr402 PAK-1,2,3 antibody. The immunolabeling of PAK is completely eliminated by treatment with λ-Ptase.
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