anti-Pan B-Cells antibody [CVS36]
![anti-Pan B-Cells antibody [CVS36]](/upload/image/products/ARG23248_FACS_1_210_205.jpg)
![anti-Pan B-Cells antibody [CVS36]](/upload/image/products/ARG23248_FACS_1.jpg)
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG23248
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [CVS36] recognizes Pan B-Cells Mouse anti Horse Pan B-Cells, clone CVS36 is a monoclonal antibody directed against equine Ig light chains. Characterization studies demonstrated that clone CVS36 bound 100% of CD5-ve peripheral blood lymphocytes and recognized all equine B-cells (Lunn et al. 1998) As the antigen recognized by clone CVS36 appears to be present on the surface of all equine B-cells it is therefore is a reagent that can be used as a pan B-cell marker for domestic horses (Breathnach et al. 2005). Specific anti equine reagents have yet to be fully characterized for the typically recognized B-cell makers such as CD19, CD20, CD21, CD22 and CD79. While testing has demonstrated the cross reactivity of some monoclonal B-cell markers raised against other species with equine B cells, such as the Mouse anti Human CD79a antibody, (clone HM57), an overview of which may be found in the report of the second equine leucocyte antigen workshop (Lunn et al. 1998). With specificity for equine Ig light chains, clone CVS36 may be used to detect all equine immunoglobulin classes and subclasses in ELISA applications (Lunn et al. 1998). |
|---|---|
| 反应物种 | Hrs |
| 应用 | ELISA, FACS |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CVS36 |
| 同位型 | IgG1 |
| 靶点名称 | Pan B-Cells |
| 抗原物种 | Horse |
| 抗原 | Purified Equine Ig. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS and 0.09% Sodium azide. |
| 抗菌剂 | 0.09% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1)
ARG23248 anti-Pan B-Cells antibody [CVS36] FACS image
Flow Cytometry: Horse peripheral blood lymphocytes stained with ARG23248 anti-Pan B-Cells antibody [CVS36] followed by Goat anti Mouse IgG (FITC).
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