anti-Parvalbumin antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Parvalbumin
  • 反应物种: Hu, Rat
  • 应用: FACS, IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Parvalbumin
  • 抗原物种: Human
  • 抗原: C-GVDEFSTLVAES
  • Brand:
CAT.NO. : ARG63769
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Parvalbumin
反应物种Hu, Rat
应用FACS, IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称Parvalbumin
抗原物种Human
抗原C-GVDEFSTLVAES
偶联标记Un-conjugated
別名PVALB; Parvalbumin; Parvalbumin Alpha; D22S749
应用说明
应用建议
应用推荐稀释比
FACS10 µg/ml
IHC-P2.5 µg/ml
WB0.1 - 0.3 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 25269 Rat PVALB

GeneID: 5816 Human PVALB

Swiss-port # P02625 Rat Parvalbumin alpha

Swiss-port # P20472 Human Parvalbumin alpha

基因名称PVALB
全名Parvalbumin
背景介绍The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Alternative splicing results in multiple transcript variants.
生物功能In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
研究领域Neuroscience antibody
预测分子量12 kDa
翻译后修饰Acetylation, Phosphoprotein
检测图片 (4)
  • ARG63769 anti-Parvalbumin antibody WB image

    Western Blot: adenovirus-mediated gene transfer in rat heart cells 1) Untransfected, 2) transient transfection with Human PVALB. 3) Untransfected rat skeletal muscle. stained with ARG63769 anti-Parvalbumin antibody at 0.15 µg/ml dilution.

  • ARG63769 anti-Parvalbumin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63769 anti-Parvalbumin antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG63769 anti-Parvalbumin antibody WB image

    Western blot: 35 µg of Human cerebellum lysate (in RIPA buffer) stained with ARG63769 anti-Parvalbumin antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63769 anti-Parvalbumin antibody FACS image

    Flow Cytometry: Paraformaldehyde-fixed Kelly cells permeabilized with 0.5% Triton. Cells were stained with ARG63769 anti-Parvalbumin antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).

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