anti-Perilipin 1 antibody
CAT.NO. : ARG64173
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概述
产品描述 | Goat Polyclonal antibody recognizes Perilipin 1 |
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反应物种 | Hu |
应用 | IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Perilipin 1 |
抗原物种 | Human |
抗原 | C-PREKPKRRVSDS |
偶联标记 | Un-conjugated |
別名 | Perilipin-1; PERI; Lipid droplet-associated protein; PLIN; FPLD4 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009] |
研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
预测分子量 | 56 kDa |
翻译后修饰 | Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet (By similarity). |
检测图片 (2)
ARG64173 anti-Perilipin 1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64173 anti-Perilipin 1 antibody at 2 µg/ml dilution followed by AP-staining.
ARG64173 anti-Perilipin 1 antibody WB image
Western blot: 35 µg of Human Adipose lysate (in RIPA buffer) stained with ARG64173 anti-Perilipin 1 antibody at 0.01 µg/ml dilution and incubated at RT for 1 hour.
