anti-PFKM antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Recombinant Rabbit Monoclonal antibody recognizes PFKM |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Monoclonal |
| 同位型 | IgG |
| 靶点名称 | PFKM |
| 抗原物种 | Human |
| 抗原 | Recombinant protein of Human PFKM. |
| 偶联标记 | Un-conjugated |
| 別名 | PFK-A; 6-phosphofructokinase type A; PPP1R122; PFKX; ATP-dependent 6-phosphofructokinase, muscle type; EC 2.7.1.11; Phosphofructo-1-kinase isozyme A; PFK1; ATP-PFK; GSD7; PFK-1; Phosphohexokinase; PFK-M; PFKA |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 阳性对照 | PC-3M | ||||||||||
| 实际分子量 | ~ 82 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | TBS (pH 7.4), 0.05% Sodium azide, 40% Glycerol and 0.05% BSA. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 40% Glycerol and 0.05% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | PFKM |
| 全名 | phosphofructokinase, muscle |
| 背景介绍 | Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009] |
| 生物功能 | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | GlcNAcylation decreases enzyme activity. [UniProt] |
ARG42606 anti-PFKM antibody ICC/IF image
Immunofluorescence: SH-SY5Y cells were fixed in paraformaldehyde and permeabilized with 0.25% Triton X100/PBS. Cells were stained with ARG42606 anti-PFKM antibody (green). DAPI (blue) for nuclear staining.
ARG42606 anti-PFKM antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse skeletal muscle tissue stained with ARG42606 anti-PFKM antibody and counter stained with hematoxylin.
ARG42606 anti-PFKM antibody WB image
Western blot: PC-3M cell lysate stained with ARG42606 anti-PFKM antibody at 1:500 dilution.
ARG42606 anti-PFKM antibody FACS image
Flow Cytometry: SH-SY5Y cells stained with ARG42606 anti-PFKM antibody (right histogram) at 1:100 dilution or without primary antibody as control (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
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