anti-PGA5 antibody [SQab18120]

CAT.NO. : ARG66418

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Size:

100 μg

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [SQab18120] recognizes PGA5
反应物种	Hu, Ms, Pig
应用	ELISA, FACS, ICC/IF, IHC-P, WB
宿主	Mouse
克隆	Monoclonal
克隆号	SQab18120
同位型	IgG1, kappa
靶点名称	PGA5
抗原物种	Human
抗原	Recombinant Human PGA5 protein.
偶联标记	Un-conjugated
別名	EC 3.4.23.1; Pepsin A-5; Pg5; Pepsinogen-5

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

纯化	Affinity purification with immunogen.
缓冲液	PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂	0.01% Thimerosal
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 5222 Human PGA5 Swiss-port # P0DJD9 Human Pepsin A-5
基因名称	PGA5
全名	pepsinogen 5, group I (pepsinogen A)
背景介绍	This gene encodes a protein precursor of the digestive enzyme pepsin, a member of the peptidase A1 family of endopeptidases. The encoded precursor is secreted by gastric chief cells and undergoes autocatalytic cleavage in acidic conditions to form the active enzyme, which functions in the digestion of dietary proteins. This gene is found in a cluster of related genes on chromosome 11, each of which encodes one of multiple pepsinogens. Pepsinogen levels in serum may serve as a biomarker for atrophic gastritis and gastric cancer. [provided by RefSeq, Jul 2015]
生物功能	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. [UniProt]
细胞定位	Secreted. [UniProt]
预测分子量	42 kDa

检测图片 (4)

ARG66418 anti-PGA5 antibody [SQab18120] ICC/IF image
Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66418 anti-PGA5 antibody [SQab18120] (green) at 1:100 dilution and cell nuclei were stained with Hoechst 33342 (blue).
ARG66418 anti-PGA5 antibody [SQab18120] FACS image
Flow Cytometry: K562 cells were stained with ARG66418 anti-PGA5 antibody [SQab18120] at 2 µg/ml (red) and without antibody control (black).
ARG66418 anti-PGA5 antibody [SQab18120] WB image
Western blot: 25 ng of Purified Human pepsinogen I, 25 ng of Purified Human pepsinogen II, 30 µg of Mouse stomach lysate and 10 µl of Human gastric juice stained with ARG66418 anti-PGA5 antibody [SQab18120] at 1:5000 dilution.
ARG66418 anti-PGA5 antibody [SQab18120] ELISA image
ELISA: The specificity of ARG66418 anti-PGA5 antibody [SQab18120] recognize recombinant proteins. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue: Pepsinogen II; Black: BSA (negative control).

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