anti-PGAM1 antibody [1G4]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [1G4] recognizes PGAM1
  • 反应物种: Hu
  • 应用: WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 1G4
  • 同位型: IgG2a, lambda
  • 靶点名称: PGAM1
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG57141
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Product Details
概述
产品描述Mouse Monoclonal antibody [1G4] recognizes PGAM1
反应物种Hu
应用WB
宿主Mouse
克隆Monoclonal
克隆号1G4
同位型IgG2a, lambda
靶点名称PGAM1
抗原物种Human
抗原Recombinant fragment around aa. 1-254 of Human PGAM1
偶联标记Un-conjugated
別名PGAMA; EC 3.1.3.13; Phosphoglycerate mutase isozyme B; HEL-S-35; EC 5.4.2.11; PGAM-B; BPG-dependent PGAM 1; Phosphoglycerate mutase 1; EC 5.4.2.4
应用说明
应用建议
应用推荐稀释比
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein A.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 5223 Human PGAM1

Swiss-port # P18669 Human Phosphoglycerate mutase 1

基因名称PGAM1
全名phosphoglycerate mutase 1 (brain)
背景介绍Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]
生物功能Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. [UniProt]
预测分子量29 kDa
翻译后修饰Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme.
检测图片 (1)
  • ARG57141 anti-PGAM1 antibody [1G4] WB image

    Western blot: 40 µg of 1) 293T, 2) Jurkat, 3) Raji, 4) A431, and 5) HeLa cell lysates stained with ARG57141 anti-PGAM1 antibody [1G4] at 1:1000.

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