Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody [Clone RM216]; H2A/H4S1p; Histone H2A/H4 (S1 phosphorylation); 31-1092-00; 31-1092-00-S
![Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody [Clone RM216]; H2A/H4S1p; Histone H2A/H4 (S1 phosphorylation); 31-1092-00; 31-1092-00-S](/upload/image/products/Fig-2_RM216_e.png)
![Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody [Clone RM216]; H2A/H4S1p; Histone H2A/H4 (S1 phosphorylation); 31-1092-00; 31-1092-00-S](/upload/image/products/Fig3_ICC_H2AH4S1p.png)
![Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody [Clone RM216]; H2A/H4S1p; Histone H2A/H4 (S1 phosphorylation); 31-1092-00; 31-1092-00-S](/upload/image/products/Fig-1-RM216_e.png)
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CAT.NO. : 31-1092-00
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Product Details
Product Details
| Product Name | Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody, Clone RM216 |
| Product Description | Rabbit monoclonal to Phospho-Histone H2A/H4 (pSer1), H2A/H4S1p, Histone H2A/H4 (S1 phosphorylation) |
| Catalog No. | 31-1092-00 |
| Clone Name | RM216 |
| Product Data Sheet | Anti-Phospho-Histone H2A/H4 (pSer1) Rabbit Monoclonal Antibody Data Sheet RM216 |
| Specificity | RM216 reacts to Histone H4 or Histone H2A phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones. |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H2A (pSer1). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ICC: 1 μg/mL - 2 μg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 1 µg/mL. |
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Product Images
 | RM216 specifically reacts to both Histone H2A and H4 phosphorylated at Serine 1 (H2AS1p and H4S1p). |
 | Western Blot of acid extracts of HeLa cells treated (1) or non-treated (2) with Nocodazole. Using RM216 at 0.5 µg/mL, showed both Histone H2A and H4 phosphorylated at Serine 1 in HeLa cells. |
 | Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
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