Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody; Histone H2B (Phospho Ser14) [Clone RM238], 31-1115-00
![Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody; Histone H2B (Phospho Ser14) [Clone RM238], 31-1115-00](/upload/image/products/Fig2_ICC_H2BS14p.png)
![Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody; Histone H2B (Phospho Ser14) [Clone RM238], 31-1115-00](/upload/image/products/Fig1_WB_H2BS14p.png)
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CAT.NO. : 31-1115-00
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Product Details
Product Details
| Product Name | Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody, Clone RM238 |
| Product Description | Rabbit monoclonal to Phospho-Histone H2B (Ser14), H2BS14p, Histone H2B (S14 phosphorylation) |
| Catalog No. | 31-1115-00 |
| Clone Name | RM238 |
| Product Data Sheet | Anti-Phospho-Histone H2B (Ser14) Rabbit Monoclonal Antibody Data Sheet RM238 |
| Specificity | RM238 reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones. |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H2B (Ser14). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ICC: 1 μg/mL - 2 μg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL - 1 µg/mL. |
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Product Images
 | Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM238 at 0.5 µg/mL, showed a band of Histone H2B phosphorylated at Serine 14 in HeLa cells. |
 | Immunocytochemistry of HeLa cells using Anti-Phospho-Histone H2B (Ser14) Rabbit mAb RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green). |
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