Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody [Clone RM163]; H3S10p; Histone H3 (S10 phosphorylation); 31-1070-00; 31-1070-00-S
![Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody [Clone RM163]; H3S10p; Histone H3 (S10 phosphorylation); 31-1070-00; 31-1070-00-S](/upload/image/products/Fig1_RM163_WB.png)
![Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody [Clone RM163]; H3S10p; Histone H3 (S10 phosphorylation); 31-1070-00; 31-1070-00-S](/upload/image/products/RM163_ICC.png)
Key features and details
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CAT.NO. : 31-1070-00
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Product Details
Product Details
| Product Name | Anti-Phospho-Histone H3 (Ser10) Rabbit Monoclonal Antibody, Clone RM163 |
| Product Description | Rabbit monoclonal to Phospho-Histone H3 (Ser10), Histone H3 (S10 phosphorylation) |
| Catalog No. | 31-1070-00 |
| Clone Name | RM163 |
| Product Data Sheet | Anti-Phospho-Histone H3 (pSer10) Rabbit Monoclonal Antibody Data Sheet RM163 |
| Specificity | RM163 reacts to Histone H3 phosphorylated at Serine 10. No cross reactivity with other phosphorylated histones. |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H3 (Ser10). |
| Application | Western Blot (WB) Immunocytochemistry (ICC) ELISA Multiplex Immunohistochemistry (IHC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ICC: 0.5 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 1 µg/mL. IHC: 0.2 µg/mL - 1 µg/mL |
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Product Images
 | Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM163 at 0.5 µg/mL, showed a band of Histone H3 phosphorylated at serine 10 in HeLa cells. |
 | Immunocytochemistry of HeLa cells using Phospho-Histone H3(Ser10) Rabbit mAb RM163 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human colon tissue section using Phospho-Histone H3 (Ser10) Rabbit mAb RM163. |
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