Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [Clone RM159]; H3pThr3; Histone H3 (Thr3 phosphorylation); 31-1072-00; 31-1072-00-S
![Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [Clone RM159]; H3pThr3; Histone H3 (Thr3 phosphorylation); 31-1072-00; 31-1072-00-S](/upload/image/products/RM159-ICC-2.png)
![Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [Clone RM159]; H3pThr3; Histone H3 (Thr3 phosphorylation); 31-1072-00; 31-1072-00-S](/upload/image/products/RM159-ICC-1.png)
![Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [Clone RM159]; H3pThr3; Histone H3 (Thr3 phosphorylation); 31-1072-00; 31-1072-00-S](/upload/image/products/RM159-Fig11.png)
![Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody [Clone RM159]; H3pThr3; Histone H3 (Thr3 phosphorylation); 31-1072-00; 31-1072-00-S](/upload/image/products/RM159_IHC-Breast-Cancer_e.png)
Key features and details
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Product Details
| Product Name | Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody, Clone RM159 |
| Product Description | Rabbit monoclonal to Phospho-Histone H3 (Thr3), Histone H3 (Thr3 phosphorylation) |
| Catalog No. | 31-1072-00 |
| Clone Name | RM159 |
| Product Data Sheet | Anti-Phospho-Histone H3 (Thr3) Rabbit Monoclonal Antibody Data Sheet RM159 |
| Specificity | RM159 reacts to Histone H3 phosphorylated at Threonine 3. No cross reactivity with other phosphorylated histones |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H3 (Thr3) |
| Application | Western Blot (WB) Immunohistochemistry (IHC) Immunocytochemistry (ICC) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.1 µg/mL - 1 µg/mL; IHC:0.5 µg/mL - 2 ug/mL; ICC:0.5 µg/mL - 2 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 1 µg/mL. |
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 | Western Blot of acid extracts of HeLa cells non-treated (1) or treated (2) with Nocodazole, and recombinant Histone H3.3 (3). Using RM159 at 0.1 µg/mL, showed a band of Histone H3 phosphorylated at threonine 3 in HeLa cells. |
 | Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Thr3) Rabbit mAb RM159 (red). Actin filaments have been labeled with fluorescein phalloidin (green). |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human Cervix Cancer tissue section using anti-Phospho-Histone H3 (Thr3) rabbit monoclonal antibody, Clone RM159, at a 0.5 µg/mL. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human Normal Colon tissue section using anti-Phospho-Histone H3 (Thr3) rabbit monoclonal antibody, Clone RM159, at a 0.5 µg/mL. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue section using anti-Phospho-Histone H3 (Thr3) rabbit monoclonal antibody, Clone RM159, at a 1 µg/mL. |
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