anti-Phosphoserine antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 靶点名称:
- 抗原:
- 偶联标记:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes Phosphoserine |
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| 反应物种 | Other |
| 应用 | ELISA, ICC/IF, IHC, IP, WB |
| 特异性 | Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 靶点名称 | Phosphoserine |
| 抗原 | KLH-conjugated Phosphoserine, and phosvitin mixture |
| 偶联标记 | Un-conjugated |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Rabbit immunoglobulin |
| 缓冲液 | PBS, 50% Glycerol and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 250 µg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 背景介绍 | Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders. |
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| 研究领域 | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
ARG20543 anti-Phosphoserine antibody ICC/IF image
Immunofluorescence: HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20543 anti-Phosphoserine antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20543 anti-Phosphoserine antibody. Right: Composite.
ARG20543 anti-Phosphoserine antibody WB image
Western blot: the phosphorylated proteins with UV-treated cell lysates (mouse spleen cell) stained with ARG20543 anti-Phosphoserine antibody. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1 μM okadaic acid).
Switch of phosphorylation to O-GlcNAcylation of AhR contributes to vascular oxidative stress induced by benzo[a]pyrene
WB/IP / Mouse
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