Anti-Phosphothreonine Rabbit Monoclonal Antibody [Clone RM102]; 31-1009-00
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Key features and details
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CAT.NO. : 31-1009-00
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Product Details
Product Details
| Product Name | Anti-Phosphothreonine Rabbit Monoclonal Antibody, Clone RM102 |
| Description | Rabbit monoclonal to Phosphothreonine |
| Catalog No. | 31-1009-00 |
| Clone Name | RM102 |
| Product Data Sheet | Anti-Phosphothreonine Rabbit Monoclonal Antibody Data Sheet RM102 |
| Specificity | RM102 reacts threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides. |
| Immunogen | Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide |
| Application | Western Blot (WB) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | All Species |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot (WB): 1:500 -1:2000 dilution; Immunoprecipitation (IP): 1:100-1:500 dilution; Chromatin IP (ChIP): 1:100-1:500 dilution; Immunocytochemistry (ICC): 1:100-1:500 dilution; Immunohistochemistry (IHC): 1:100-1:500 dilution. |
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Product Images
 | Immunocytochemistry of serum-starved A431 cells nontreated or treated with Calyculin A/Okadaic Acid, using RevMAb Clone RM102 at 1/500 dilution (followed by PE conjugated secondary antibody, red) and DAPI (blue). |
 | Western blot of serum-starved A431 cells non-treated or treated with Calyculin A/Okadaic Acid, using RevMAb Clone RM102 at 1/2000 dilution. |
 | Immunoprecipitation of Calyculin A/Okadaic Acid treated A431 cells by RevMAb Clone RM102 at 1/500 dilution, and then blotted with RM102. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by RM102. |
 | RevMAb rabbit monoclonal antibody, Clone RM102, recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine. |
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