anti-Phosphotyrosine antibody [P-TYR-01]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [P-TYR-01] recognizes Phosphotyrosine
  • 反应物种: All
  • 应用: FACS, ICC/IF, WB
  • 特异性: The clone P-Tyr-01 detects tyrosine phosphorylation in activated cells.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: P-TYR-01
  • 同位型: IgG1
  • 靶点名称: Phosphotyrosine
  • Brand:
CAT.NO. : ARG63086
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Product Details
概述
产品描述Mouse Monoclonal antibody [P-TYR-01] recognizes Phosphotyrosine
反应物种All
应用FACS, ICC/IF, WB
特异性The clone P-Tyr-01 detects tyrosine phosphorylation in activated cells.
宿主Mouse
克隆Monoclonal
克隆号P-TYR-01
同位型IgG1
靶点名称Phosphotyrosine
抗原Phosphotyrosine conjugated to bovine serum albumin.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
FACS2 - 5 µg/ml
ICC/IFAssay-dependent
WB1 - 2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from ascites by protein-A affinity chromatography.
纯度> 95% (by SDS-PAGE)
缓冲液PBS (pH 7.4) and 15 mM Sodium azide
抗菌剂15 mM Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
研究领域Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
检测图片 (1)
  • ARG63086 anti-Phosphotyrosine antibody [P-TYR-01] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG63086 anti-Phosphotyrosine antibody [P-TYR-01] (green).
    Actin was stained with phalloidin-Alexa Fluor-567 (red), DNA was stained with DAPI (blue).

参考文献

克隆号文献

Fully automated synthesis of (phospho)peptide arrays in microtiter plate wells provides efficient access to protein tyrosine kinase characterization.

Saxinger C et al.
BMC Immunol.,  (2005)

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Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.

Brdicka T et al.
J Exp Med.,  (2000)

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T cell receptor signalling results in rapid tyrosine phosphorylation of the linker protein LAT present in detergent-resistant membrane microdomains.

Brdiĉka T et al.
Biochem Biophys Res Commun.,  (1998)

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Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells.

Cebecauer M et al.
Biochem Biophys Res Commun.,  (1998)

publication_link

 

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