Anti-Phosphotyrosine Rabbit Monoclonal Antibody [Clone RM111]; 32-1011-00
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Key features and details
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Product Details
| Product Name | Anti-Phosphotyrosine Rabbit Monoclonal Antibody, Clone RM111 |
| Product Description | Rabbit monoclonal to Phosphotyrosine |
| Catalog No. | 32-1011-00 |
| Clone Name | RM111 |
| Product Data Sheet | Anti-Phosphotyrosine Rabbit Monoclonal Antibody Data Sheet RM111 |
| Specificity | RM111 reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine |
| Immunogen | Phosphotyrosine-BSA conjugate |
| Application | Western Blot (WB) Immunoprecipitation (IP) Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | All Species |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot (WB): 1:1000 -1:5000 dilution; Immunoprecipitation (IP): 1:200-1:1000 dilution; Chromatin IP (ChIP): 1:200-1:1000 dilution; Immunocytochemistry (ICC): 1:200-1:500 dilution; Immunohistochemistry (IHC): 1:200-1:500 dilution. |
| Keywords | |
| References for Phosphotyrosine Rabbit Monoclonal Antibody [RM111] | Sun Y et al. Neuregulin-1/ErbB4 Signaling Regulates Visual Cortical Plasticity Neuron (2016) 10.1016/j.neuron.2016.08.033. IHC; Mouse. Read more (PubMed: 27641496) |
 | Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, usingRevMAb Clone RM111 at 1/500 dilution (foll-owed by a PE conjugated secondary antibody, red) and DAPI (blue). |
 | Western blot of serum-starved A431 cells non-treated or treated with EGF, using RevMAb Clone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points. |
 | Immunoprecipitation of EGF-treated A431 cells by RevMAb Clone RM111 at 1/1000 dilution, was blotted with an anti-EGFR rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by rabbit IgG control; (3) IP by Clone RM111. |
 | Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. RevMAb Clone RM111 reacts only to phosphorylated Tyrosine. |
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