anti-PIK3CA / p110 alpha antibody [SP139]

Key features and details

产品描述: Rabbit Monoclonal antibody [SP139] recognizes PIK3CA / p110 alpha
反应物种: Hu
应用: IHC-P
宿主: Rabbit
克隆: Monoclonal
克隆号: SP139
同位型: IgG
靶点名称: PIK3CA / p110 alpha
抗原物种: Human

Brand:

CAT.NO. : ARG53467

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Size:

100 μl

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Rabbit Monoclonal antibody [SP139] recognizes PIK3CA / p110 alpha
反应物种	Hu
应用	IHC-P
宿主	Rabbit
克隆	Monoclonal
克隆号	SP139
同位型	IgG
靶点名称	PIK3CA / p110 alpha
抗原物种	Human
抗原	Synthetic peptide in the internal region of the human pI3KCA protein.
偶联标记	Un-conjugated
別名	MCM; MCMTC; p110alpha; PI3-kinase subunit alpha; PI3K; CWS5; p110-alpha; PI3Kalpha; CLOVE; EC 2.7.1.153; MCAP; PtdIns-3-kinase subunit p110-alpha; EC 2.7.11.1; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PI3K-alpha; PtdIns-3-kinase subunit alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA

应用说明

应用建议

应用	推荐稀释比
IHC-P	1:100

应用说明

IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

阳性对照

Breast Carcinoma

属性

形式	Liquid
纯化	Purified by protein A/G
缓冲液	PBS (pH 7.6), 1% BSA and
抗菌剂
稳定剂	1% BSA
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 5290 Human PIK3CA Swiss-port # P42336 Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha iso
基因名称	PIK3CA
全名	phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
背景介绍	Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq, Jul 2008]
生物功能	Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. [UniProt]
细胞定位	Membrane
研究领域	Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
预测分子量	124 kDa

检测图片 (8)

ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Breast Ductal Carcinoma stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Colon Adenocarcinoma stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Stomach stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Stomach Adenocarcinoma stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Bladder Transitional Cell Carcinoma stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Prostate Adenocarcinoma stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Skin Squamous Cell Carcinoma stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].
ARG53467 anti-PIK3CA / p110 alpha antibody [SP139] IHC-P image
Immunohistochemistry: Human Skin stained with ARG53467 anti-PIK3CA / p110 alpha antibody [SP139].