anti-Pirin antibody
| 产品描述 | Goat Polyclonal antibody recognizes Pirin |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms |
| 应用 | IHC-P, WB |
| 特异性 | Variants (NP_003653.1; NP_001018119.1) encode the same protein |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Pirin |
| 抗原物种 | Human |
| 抗原 | GSSKKVTLSVLSREC |
| 偶联标记 | Un-conjugated |
| 別名 | EC 1.13.11.24; Probable quercetinase; Pirin; Probable quercetin 2,3-dioxygenase PIR |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| 研究领域 | Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 32 kDa |
ARG63633 anti-Pirin antibody WB image
Western Blot: Human Heart lysate (35 µg protein in RIPA buffer) stained with ARG63633 anti-Pirin antibody at 0.3 µg/ml dilution.
ARG63633 anti-Pirin antibody WB image
Western Blot: 1). Mock transfection; 2) PIR (RC223111) expressing plasmid transfected HEK293 cell lysate standed with ARG63633 anti-Pirin antibody
ARG63633 anti-Pirin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63633 anti-Pirin antibody at 5 µg/ml dilution followed by AP-staining.
ARG63633 anti-Pirin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human spleen tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63633 anti-Pirin antibody at 5 µg/ml dilution followed by AP-staining.
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