anti-PLA2G4A antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes PLA2G4A |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLA2G4A |
| 抗原物种 | Human |
| 抗原 | C-QEKTFRQQRKEHIR |
| 偶联标记 | Un-conjugated |
| 別名 | Phospholipase A2 group IVA; cPLA2; cPLA2-alpha; Phosphatidylcholine 2-acylhydrolase; PLA2G4; Cytosolic phospholipase A2; EC 3.1.1.5; EC 3.1.1.4 |
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 85 kDa |
| 翻译后修饰 | Activated by phosphorylation at both Ser-505 and Ser-727. |
ARG65251 anti-PLA2G4A antibody WB image
Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG65251 anti-PLA2G4A antibody at 1 µg/ml dilution.
ARG65251 anti-PLA2G4A antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A549 cells permeabilized with 0.15% Triton. Cells were stained with ARG65251 anti-PLA2G4A antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
ARG65251 anti-PLA2G4A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65251 anti-PLA2G4A antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG65251 anti-PLA2G4A antibody WB image
Western blot: 35 µg of A549 cell lysate (in RIPA buffer) stained with ARG65251 anti-PLA2G4A antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
ARG65251 anti-PLA2G4A antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A549 cells permeabilized with 0.5% Triton. Cells were stained with ARG65251 anti-PLA2G4A antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
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WB / Mouse
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