anti-PLN / Phospholamban phospho (Thr17) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG41951
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PLN / Phospholamban phospho (Thr17) |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | Dot, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PLN / Phospholamban |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated phosphospecific peptide around Thr17 of Human PLN. |
| 偶联标记 | Un-conjugated |
| 別名 | CMH18; PLB; CMD1P; Cardiac phospholamban |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | Spontaneous hypertensive rat heart | ||||||
| 实际分子量 | 11 and 26 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide. |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PLN |
| 全名 | phospholamban |
| 背景介绍 | The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008] |
| 生物功能 | Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Single-pass membrane protein. Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Membrane; Single-pass membrane protein. Note=Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). [UniProt] |
| 预测分子量 | 6 kDa |
| 翻译后修饰 | Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. [UniProt] |
检测图片 (2)
ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody WB image
Western blot: Spontaneous hypertensive (SH) rat heart and normal rat heart tissue lysates stained with ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody.
ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody Dot image
Dot blot: Analysis of ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody on nitrocellulose membrane. 50 ng of Phosphopeptide or Non-phosphopeptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.
参考文献
Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats
WB / Rat
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