anti-POLL / POLkappa antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes POLL / POLkappa
  • 反应物种: Hu
  • 应用: WB
  • 特异性: It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus.Further the protein structures appear to be very similar in this region.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: POLL / POLkappa
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63382
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes POLL / POLkappa
反应物种Hu
应用WB
特异性It has been brought to our attention that this product may crossreact with Pol beta (NP_002681), an evolutionarilly related polymerase with a predicted size of 39 kDa. The overall % of identity is not very high (about 32% in the core) but considering only the peptide that we used as immunogen the two share the sequence YREP**R** at the C-terminus.Further the protein structures appear to be very similar in this region.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称POLL / POLkappa
抗原物种Human
抗原C-LGLPYREPAERDW
偶联标记Un-conjugated
別名DNA polymerase beta-2; BETAN; EC 4.2.99.-; EC 2.7.7.7; Pol beta2; Pol Lambda; POLKAPPA; DNA polymerase kappa; DNA polymerase lambda
应用说明
应用建议
应用推荐稀释比
WB0.5 - 1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 27343 Human POLL

Swiss-port # Q9UGP5 Human DNA polymerase lambda

背景介绍This gene encodes a DNA polymerase. DNA polymerases catalyze DNA-template-directed extension of the 3'-end of a DNA strand. This particular polymerase, which is a member of the X family of DNA polymerases, likely plays a role in non-homologous end joining and other DNA repair processes. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2010]
研究领域Gene Regulation antibody
预测分子量63 kDa
检测图片 (1)
  • ARG63382 anti-POLL / POLkappa antibody WB image

    Western blot: 35 µg of A549 (A) and HepG2 (B) cell lysates (in RIPA buffer) stained with ARG63382 anti-POLL / POLkappa antibody at 0.5 µg/ml (A) and 1 µg/ml (B) dilutions and incubated at RT for 1 hour.

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