anti-PPP2R1A antibody
产品描述 | Goat Polyclonal antibody recognizes PPP2R1A |
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反应物种 | Hu |
预测物种 | Ms, Rat, Cow, Dog, Pig |
应用 | IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | PPP2R1A |
抗原物种 | Human |
抗原 | C-KYFAQEALTVLSLA |
偶联标记 | Un-conjugated |
別名 | Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform R1-alpha; PP2A-Aalpha; PR65A; PP2A subunit A isoform PR65-alpha; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; MRD36; PP2AAALPHA |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | Swiss-port # P30153 Human Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha |
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背景介绍 | This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
研究领域 | Signaling Transduction antibody |
预测分子量 | 65 kDa |
ARG63740 anti-PPP2R1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human heart tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63740 anti-PPP2R1A antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG63740 anti-PPP2R1A antibody IHC image
Immunohistochemistry: paraffin-embedded Human Thyroid Gland (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63740 anti-PPP2R1A antibody at 3.8 µg/ml dilution, followed by AP-staining.
ARG63740 anti-PPP2R1A antibody WB image
Western blot: 35 µg of Human thymus (A) and Human lymph node (B) lysates (in RIPA buffer) stained with ARG63740 anti-PPP2R1A antibody at 2 µg/ml dilution and incubated at RT for 1 hour.
