anti-PRDM1 / Blimp 1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes PRDM1 / Blimp 1
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: PRDM1 / Blimp 1
  • 抗原物种: Human
  • 抗原: E. coli-derived Human PRDM1/Blimp1 recombinant protein (Position: M1-Q230).
  • Brand:
CAT.NO. : ARG58294
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes PRDM1 / Blimp 1
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称PRDM1 / Blimp 1
抗原物种Human
抗原E. coli-derived Human PRDM1/Blimp1 recombinant protein (Position: M1-Q230).
偶联标记Un-conjugated
別名Beta-interferon gene positive regulatory domain I-binding factor; PR domain zinc finger protein 1; BLIMP-1; BLIMP1; PRDI-binding factor 1; PR domain-containing protein 1; EC 2.1.1.-; PRDI-BF1; Positive regulatory domain I-binding factor 1
应用说明
应用建议
应用推荐稀释比
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 12142 Mouse PRDM1

GeneID: 639 Human PRDM1

Swiss-port # O75626 Human PR domain zinc finger protein 1

Swiss-port # Q60636 Mouse PR domain zinc finger protein 1

基因名称PRDM1
全名PR domain containing 1, with ZNF domain
背景介绍This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]
生物功能Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells. [UniProt]
细胞定位Nucleus. [UniProt]
预测分子量92 kDa
翻译后修饰Sumoylation at Lys-816 by PIAS1 augments transcriptional repressor activity, and is critical for plasma cell differentiation.

Ubiquitinated by the SCF(FBXO11) complex, leading to its degradation by the proteasome. [UniProt]
检测图片 (8)
  • ARG58294 anti-PRDM1 / Blimp 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody WB image

    Western blot: 50 µg of Rat thymus, Rat spleen, Rat stomach, Rat lung, Mouse thymus, Mouse spleen, Mouse stomach, Mouse lung and Mouse HEPA1-6 whole cell lysates stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat small intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG58294 anti-PRDM1 / Blimp 1 antibody WB image

    Western blot: 50 µg of HeLa whole cell lysates, A549 whole cell lysates, 293T whole cell lysates, MCF-7 whole cell lysates and 22RV1 whole cell lysates stained with ARG58294 anti-PRDM1 / Blimp 1 antibody at 0.5 µg/ml, overnight at 4°C, under reducing conditions.

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