anti-Prohibitin antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes Prohibitin |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Hm |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Prohibitin |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence at the C-terminus of Human Prohibitin (240-256aa KLEAAEDIAYQLSRSRN) |
| 偶联标记 | Un-conjugated |
| 別名 | HEL-215; PHB1; Prohibitin; HEL-S-54e |
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| 抗菌剂 | 0.05% Thimerosal and 0.05% Sodium azide |
| 稳定剂 | 5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | PHB |
| 全名 | prohibitin |
| 背景介绍 | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| 生物功能 | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. [UniProt] |
| 细胞定位 | Mitochondrion inner membrane. [UniProt] |
| 预测分子量 | 30 kDa |
ARG40831 anti-Prohibitin antibody ICC image
Immunocytochemistry: HeLa cells stained with ARG40831 anti-Prohibitin antibody.
ARG40831 anti-Prohibitin antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG40831 anti-Prohibitin antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG40831 anti-Prohibitin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG40831 anti-Prohibitin antibody.
ARG40831 anti-Prohibitin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40831 anti-Prohibitin antibody at 1 µg/ml dilution, overnight at 4°C.
ARG40831 anti-Prohibitin antibody WB image
Western blot: Rat lung, Rat skeletal muscle, Rat brain, Rat kidney, HeLa, MCF7, PC-12, A549 and SMMC-7721 cell lysates stained with ARG40831 anti-Prohibitin antibody.
ARG40831 anti-Prohibitin antibody WB image
Western blot: 50 µg of sample under reducing conditions. NIH/3T3 whole cell lysate stained with ARG40831 anti-Prohibitin antibody at 0.5 µg/ml dilution, overnight at 4°C.
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