anti-PRR7 antibody [TRAP3/10]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
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Brand:
CAT.NO. : ARG53960
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [TRAP3/10] recognizes PRR7 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 特异性 | The clone TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | TRAP3/10 |
| 同位型 | IgG2a |
| 靶点名称 | PRR7 |
| 抗原物种 | Human |
| 抗原 | Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253) |
| 偶联标记 | Un-conjugated |
| 別名 | Synaptic proline-rich membrane protein; Proline-rich protein 7 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | ICC/IF: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | WB: Murine brain lysate (red. Laemmli buffer) |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRR7 |
| 全名 | proline rich 7 (synaptic) |
| 背景介绍 | PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse. |
| 研究领域 | Neuroscience antibody |
| 预测分子量 | 31 kDa |
检测图片 (2)
ARG53960 anti-PRR7 antibody [TRAP3/10] ICC/IF image
Immunofluorescence: HEK-293 cells cotransfected with PRR7 / TRAP3 and GFP-PSD-95 stained with ARG53960 anti-PRR7 antibody [TRAP3/10] (red)
Co-stained with GFP (green). Cell nuclei was stained with DAPI (blue).ARG53960 anti-PRR7 antibody [TRAP3/10] WB image
Western blot: Murine brain lysate stained with ARG53960 anti-PRR7 antibody [TRAP3/10].
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