anti-PSMF1 antibody
CAT.NO. : ARG63253
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概述
产品描述 | Goat Polyclonal antibody recognizes PSMF1 |
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反应物种 | Hu |
预测物种 | Ms, Rat, Cow, Dog |
应用 | WB |
特异性 | This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2). |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | PSMF1 |
抗原物种 | Human |
抗原 | C-DHLPPPGYDDMYL |
偶联标记 | Un-conjugated |
別名 | hPI31; PI31; Proteasome inhibitor PI31 subunit |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. Alternative transcript variants have been identified for this gene. [provided by RefSeq, Jul 2008] |
研究领域 | Cell Biology and Cellular Response antibody |
预测分子量 | 30 kDa |
检测图片 (2)
ARG63253 anti-PSMF1 antibody WB image
Western blot: Human Kidney lysate (RIPA buffer, 30 µg total protein per lane) stained with ARG63253 anti-PSMF1 antibody at 1 µg/ml dilution.
ARG63253 anti-PSMF1 antibody WB image
Western blot: 1). Mock transfection; 2) PSMF1 (RC218938) expressing plasmid transfected HEK293 cell lysate standed with ARG63253 anti-PSMF1 antibody.
