anti-PTPN3 / PTPH1 phospho (Ser459) antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG52405
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes PTPN3 / PTPH1 phospho (Ser459) |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Bov, Dog, NHuPrm, Sheep |
| 应用 | WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | PTPN3 / PTPH1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic phospho-peptide around Ser459. |
| 偶联标记 | Un-conjugated |
| 別名 | PTPH1; EC 3.1.3.48; Protein-tyrosine phosphatase H1; Tyrosine-protein phosphatase non-receptor type 3; PTP-H1 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | Specific for ~104k PTPH1 protein phosphorylated at Ser459 . Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with λ phosphatase. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity Purified |
| 缓冲液 | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| 稳定剂 | 0.1 mg/ml BSA, 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # A2ALK8 Mouse Tyrosine-protein phosphatase non-receptor type 3 Swiss-port # P26045 Human Tyrosine-protein phosphatase non-receptor type 3 |
|---|---|
| 基因名称 | PTPH1 |
| 全名 | protein tyrosine phosphatase, non-receptor type 3 |
| 背景介绍 | Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012). |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 104 kDa |
检测图片 (1)
ARG52405 anti-PTPN3 / PTPH1 phospho (Ser459) antibody WB image
Western blot: Mouse testes lysate showing specific immunolabeling of ~104 kDa PTPH1 phosphorylated at Ser459 (Control).
Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase) stained with ARG52405 anti-PTPN3 / PTPH1 phospho (Ser459) antibody.
The blot is identical to the control except that the lysate was incubated in λ-Ptase (400 units/100 µl lysate for 30 min, RT) before being exposed to the phospho-Ser 459 PTPN3 / PTPH1 antibody.
The immunolabeling is completely eliminated by treatment with λ-Ptase.
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