anti-PUM2 antibody
CAT.NO. : ARG63763
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概述
产品描述 | Goat Polyclonal antibody recognizes PUM2 |
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反应物种 | Hu |
预测物种 | Cow |
应用 | IHC-P, WB |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | PUM2 |
抗原物种 | Human |
抗原 | C-DQNRDLKQGDDDDSK |
偶联标记 | Un-conjugated |
別名 | PUML2; Pumilio-2; PUMH2; Pumilio homolog 2 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | |
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背景介绍 | This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
研究领域 | Developmental Biology antibody; Gene Regulation antibody |
预测分子量 | 114 kDa |
检测图片 (2)
ARG63763 anti-PUM2 antibody WB image
Western blot: 10 µg of HEK293 cell lysate (in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag stained with ARG63763 anti-PUM2 antibody at 0.5 µg/ml dilution (Lane A); Mock-transfected HEK293 stained with primary antibodies (Lane B); Overexpressing HEK293 cell lysate stained with anti-DYKDDDDK Tag at 1:3000 dilution (Lane C). Primary antibodies were incubated at RT for 1 hour.
ARG63763 anti-PUM2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63763 anti-PUM2 antibody at 10 µg/ml dilution followed by AP-staining.
