anti-RAB35 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes RAB35
  • 反应物种: Hu, Ms
  • 预测物种: Bov, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: RAB35
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG66777
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes RAB35
反应物种Hu, Ms
预测物种Bov, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称RAB35
抗原物种Human
抗原KLH-conjugated synthetic peptide within the center region of Human RAB35.
偶联标记Un-conjugated
別名Ras-related protein Rab-1C; RAB1C; Ras-related protein Rab-35; H-ray; GTP-binding protein RAY; RAY
应用说明
应用建议
应用推荐稀释比
IHC-P1:100
WB1:500 - 1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照MCF7 and Raw264.7
实际分子量~ 23 kDa
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol.
抗菌剂0.01% Sodium azide
稳定剂30% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 11021 Human RAB35

GeneID: 77407 Mouse RAB35

Swiss-port # Q15286 Human Ras-related protein Rab-35

Swiss-port # Q6PHN9 Mouse Ras-related protein Rab-35

基因名称RAB35
全名RAB35, member RAS oncogene family
生物功能The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes. [UniProt]
细胞定位Cell membrane; Lipid-anchor. Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome. Note=Present on sorting endosomes and recycling endosome tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified in stage I to stage IV melanosomes. [UniProt]
预测分子量23 kDa
翻译后修饰AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB35 constitutively active.

Phosphocholinated by L.pneumophila AnkX. Both GDP-bound and GTP-bound forms can be phosphocholinated. Phosphocholination inhibits the GEF activity of DENND1A. [UniProt]
检测图片 (2)
  • ARG66777 anti-RAB35 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human liver tissue stained with ARG66777 anti-RAB35 antibody.

  • ARG66777 anti-RAB35 antibody WB image

    Western blot: MCF7 and Raw264.7 whole cell lysates stained with ARG66777 anti-RAB35 antibody.

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