anti-RAGE antibody [SQab1745]

Key features and details

产品描述: Mouse Monoclonal antibody [SQab1745] recognizes RAGE
反应物种: Hu, Ms, Rat
应用: ELISA, FACS, ICC/IF, IHC, WB
宿主: Mouse
克隆: Monoclonal
克隆号: SQab1745
同位型: IgG1
靶点名称: RAGE
抗原物种: Human

Brand:

CAT.NO. : ARG66282

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Size:

100 μg

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*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [SQab1745] recognizes RAGE
反应物种	Hu, Ms, Rat
应用	ELISA, FACS, ICC/IF, IHC, WB
宿主	Mouse
克隆	Monoclonal
克隆号	SQab1745
同位型	IgG1
靶点名称	RAGE
抗原物种	Human
抗原	Recombinant Human RAGE protein.
偶联标记	Un-conjugated
別名	Receptor for advanced glycosylation end products; Advanced glycosylation end product-specific receptor; RAGE

应用说明

应用建议

应用	推荐稀释比
ELISA	1:3000 - 1:10000
FACS	1:400 - 1:1000
ICC/IF	1:400 - 1:1000
IHC	Assay-dependent
WB	1:2500 - 1:5000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
缓冲液	PBS (pH 7.4) and 0.01% Thimerosal.
抗菌剂	0.01% Thimerosal
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 177 Human AGER Swiss-port # Q15109 Human Advanced glycosylation end product-specific receptor
基因名称	AGER
全名	advanced glycosylation end product-specific receptor
背景介绍	RAGE: Advanced glycosylation end product (AGE) receptor is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]
生物功能	RAGE Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides. [UniProt]
产品亮点	Related products: RAGE antibodies; RAGE ELISA Kits; Anti-Mouse IgG secondary antibodies;
预测分子量	43 kDa

检测图片 (7)

ARG66282 anti-RAGE antibody [SQab1745] ICC/IF image
Immunofluorescence: 293T cells were transfected with RAGE plasmid and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66282 anti-RAGE antibody [SQab1745] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).
ARG66282 anti-RAGE antibody [SQab1745] FACS image
Flow Cytometry: 293T (black) and RAGE transfected 293T cells (red) stained with ARG66282 anti-RAGE antibody [SQab1745] at 1 µg/ml dilution.
ARG66282 anti-RAGE antibody [SQab1745] WB image
Western blot: 15 µg of 293T and RAGE transfected 293T cell lysates stained with ARG66282 anti-RAGE antibody [SQab1745] at 1:5000 dilution.
ARG66282 anti-RAGE antibody [SQab1745] ELISA image
ELISA: Titration curve of ARG66282 anti-RAGE antibody [SQab1745] in ELISA. Red: RAGE; Black: BSA (negative control).
ARG66282 anti-RAGE antibody [SQab1745] WB image
Western blot: 50 µg of Rat lung lysate stained with ARG66282 anti-RAGE antibody [SQab1745] at 1:5000 dilution.
ARG66282 anti-RAGE antibody [SQab1745] WB image
Western blot: 50 µg of Mouse lung lysate stained with ARG66282 anti-RAGE antibody [SQab1745] at 1:5000 dilution.
ARG66282 anti-RAGE antibody [SQab1745] WB image
Western blot: 30 µg of 3T3, HeLa and 293 cell lysates stained with ARG66282 anti-RAGE antibody [SQab1745] at 1:5000 dilution.