anti-Ras (pan) antibody [2G9]

CAT.NO. : ARG57108

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [2G9] recognizes Ras (pan)
反应物种	Hu
应用	FACS, WB
宿主	Mouse
克隆	Monoclonal
克隆号	2G9
同位型	IgG2a, kappa
靶点名称	Ras (pan)
抗原物种	Human
抗原	Recombinant fragment around aa. 1-186 of Human Ras (pan)
偶联标记	Un-conjugated
別名	GTPase NRas; N-ras; NRAS1; CMNS; Transforming protein N-Ras; NCMS; ALPS4; NS6

应用说明

应用建议

应用	推荐稀释比
FACS	Assay-dependent
WB	1:500 - 1:5000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 4893 Human NRAS Swiss-port # P01111 Human GTPase NRas
基因名称	NRAS
全名	neuroblastoma RAS viral (v-ras) oncogene homolog
背景介绍	This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]
生物功能	Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. [UniProt]
预测分子量	21 kDa
翻译后修饰	Palmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi. Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).

检测图片 (3)

ARG57108 anti-Ras (pan) antibody [2G9] FACS image
Flow Cytometry: HeLa cell line stained with ARG57108 anti-Ras (pan) antibody [2G9] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57108 anti-Ras (pan) antibody [2G9] WB image
Western blot: 35 µg of 293T cell lysates stained with ARG57108 anti-Ras (pan) antibody [2G9] at 1) 1:500, 2) 1:1000, and 3) 1:5000.
ARG57108 anti-Ras (pan) antibody [2G9] WB image
Western blot: 200 ng of NRAS, KRAS and HRAS recombinant proteins stained with ARG57108 anti-Ras (pan) antibody [2G9].

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