anti-Rb1 / Retinoblastoma protein antibody [1G5]
![anti-Rb1 / Retinoblastoma protein antibody [1G5]](/upload/image/20241105/8415bc38bc270b898ee2a90369a6c7fb.jpg)
Key features and details
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Brand:
CAT.NO. : ARG54423
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [1G5] recognizes Rb1 / Retinoblastoma protein |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA, IHC, IP, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1G5 |
| 同位型 | IgG1 |
| 靶点名称 | Rb1 / Retinoblastoma protein |
| 抗原物种 | Human |
| 抗原 | Recombinant retinoblastoma protein (p110 RB ) produced in E. coli. |
| 抗原表位 | 371-390 a.a. |
| 偶联标记 | Un-conjugated |
| 別名 | Rb; PPP1R130; pRb; pp110; RB; Retinoblastoma-associated protein; p105-Rb; OSRC |
应用说明
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G-purified |
| 缓冲液 | PBS (pH 7.4) |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RB1 |
| 全名 | retinoblastoma 1 |
| 背景介绍 | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008] |
| 生物功能 | Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. [UniProt] |
| 产品亮点 | Related products: Retinoblastoma protein antibodies; Retinoblastoma protein Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Senescence Marker Antibody Panel is launched |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody |
| 预测分子量 | 106 kDa |
| 翻译后修饰 | Phosphorylated by CDK6 and CDK4, and subsequently by CDK2 at Ser-567 in G1, thereby releasing E2F1 which is then able to activate cell growth. Dephosphorylated at the late M phase. SV40 large T antigen, HPV E7 and adenovirus E1A bind to the underphosphorylated, active form of pRb. Phosphorylation at Thr-821 and Thr-826 promotes interaction between the C-terminal domain C and the Pocket domain, and thereby inhibits interactions with heterodimeric E2F/DP transcription factor complexes. Dephosphorylated at Ser-795 by calcineruin upon calcium stimulation. CDK3/cyclin-C-mediated phosphorylation at Ser-807 and Ser-811 is required for G0-G1 transition. Phosphorylated by CDK1 and CDK2 upon TGFB1-mediated apoptosis (By similarity). N-terminus is methylated by METTL11A/NTM1 (By similarity). Monomethylation at Lys-810 by SMYD2 enhances phosphorylation at Ser-807 and Ser-811, and promotes cell cycle progression. Monomethylation at Lys-860 by SMYD2 promotes interaction with L3MBTL1. Acetylation at Lys-873 and Lys-874 regulates subcellular localization, at least during keratinocytes differentiation. |
参考文献
克隆号文献
Retinoblastoma protein monoclonal antibodies with novel characteristics.
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