anti-RBFOX2 / RBM9 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes RBFOX2 / RBM9
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: RBFOX2 / RBM9
  • 抗原物种: Human
  • 抗原: Recombinant protein corresponding to M1-Q81 of Human RBFOX2 / RBM9.
  • Brand:
CAT.NO. : ARG43057
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes RBFOX2 / RBM9
反应物种Hu, Ms, Rat
应用FACS, ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称RBFOX2 / RBM9
抗原物种Human
抗原Recombinant protein corresponding to M1-Q81 of Human RBFOX2 / RBM9.
偶联标记Un-conjugated
別名RNA binding protein fox-1 homolog 2; dJ106I20.3; RTA; HRNBP2; HNRBP2; Fox-1 homolog B; RBM9; Hexaribonucleotide-binding protein 2; RNA-binding protein 9; fxh; Repressor of tamoxifen transcriptional activity; Fox-2; FOX2; RNA-binding motif protein 9
应用说明
应用建议
应用推荐稀释比
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose.
抗菌剂0.05% Sodium azide
稳定剂4% Trehalose
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 23543 Human RBFOX2

GeneID: 362950 Rat RBFOX2

GeneID: 93686 Mouse RBFOX2

基因名称RBFOX2
全名RNA binding protein, fox-1 homolog (C. elegans) 2
背景介绍This gene is one of several human genes similar to the C. elegans gene Fox-1. This gene encodes an RNA binding protein that is thought to be a key regulator of alternative exon splicing in the nervous system and other cell types. The protein binds to a conserved UGCAUG element found downstream of many alternatively spliced exons and promotes inclusion of the alternative exon in mature transcripts. The protein also interacts with the estrogen receptor 1 transcription factor and regulates estrogen receptor 1 transcriptional activity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
生物功能RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Prevents binding of U2AF2 to the 3'-splice site. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis (By similarity). RNA-binding protein that seems to act as a coregulatory factor of ER-alpha. [UniProt]
细胞定位Nucleus. Cytoplasm. [UniProt]
预测分子量41 kDa
检测图片 (7)
  • ARG43057 anti-RBFOX2 / RBM9 antibody ICC/IF image

    Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG43057 anti-RBFOX2 / RBM9 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.

  • ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human melanoma tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43057 anti-RBFOX2 / RBM9 antibody WB image

    Western blot: 50 µg of sample under reducing conditions. U2OS, Caco-2, A431, U-87MG, Rat brain and Mouse brain lysates stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 0.5 µg/ml dilution, overnight at 4°C.

  • ARG43057 anti-RBFOX2 / RBM9 antibody FACS image

    Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG43057 anti-RBFOX2 / RBM9 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG43057 anti-RBFOX2 / RBM9 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG43057 anti-RBFOX2 / RBM9 antibody at 1 µg/ml dilution, overnight at 4°C.

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