anti-RBX1 / ROC1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG55861
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RBX1 / ROC1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RBX1 / ROC1 |
| 抗原物种 | Human |
| 抗原 | KLH-conjugated synthetic peptide corresponding to aa. 74-108 of Human RBX1 / ROC1. |
| 偶联标记 | Un-conjugated |
| 別名 | E3 ubiquitin-protein ligase RBX1; RING finger protein 75; EC 6.3.2.-; RNF75; Regulator of cullins 1; RING-box protein 1; ROC1; Protein ZYP; Rbx1; BA554C12.1 |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A and immunogen peptide. |
| 缓冲液 | PBS and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RBX1 |
| 全名 | ring-box 1, E3 ubiquitin protein ligase |
| 背景介绍 | This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.[provided by RefSeq, Sep 2010] |
| 生物功能 | E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus |
| 产品亮点 | Related products: RBX1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Keap1-Nrf2-ARE antibody panel is launched |
| 预测分子量 | 12 kDa |
检测图片 (3)
ARG55861 anti-RBX1 / ROC1 antibody WB image
Western blot: 20 µg of HeLa and 293T cell lysates stained with ARG55861 anti-RBX1 / ROC1 antibody at 1:1000 dilution.
ARG55861 anti-RBX1 / ROC1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG55861 anti-RBX1 / ROC1 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
ARG55861 anti-RBX1 / ROC1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse testis tissue stained with ARG55861 anti-RBX1 / ROC1 antibody at 1:100 dilution.
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