anti-Retinoic Acid Receptor alpha antibody [763]
![anti-Retinoic Acid Receptor alpha antibody [763]](/upload/image/products/ARG62618_WB_190625_210_205.jpg)
![anti-Retinoic Acid Receptor alpha antibody [763]](/upload/image/products/ARG62618_WB_190625.jpg)
Key features and details
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- 靶点名称:
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CAT.NO. : ARG62618
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [763] recognizes Retinoic Acid Receptor alpha |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 预测物种 | Bov, Dog, Gpig |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 763 |
| 同位型 | IgG1 |
| 靶点名称 | Retinoic Acid Receptor alpha |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to the N-terminal region of RARA conjugated to KLH |
| 偶联标记 | Un-conjugated |
| 別名 | Nuclear receptor subfamily 1 group B member 1; RAR; RAR-alpha; Retinoic acid receptor alpha; NR1B1 |
应用说明
| 应用说明 | WB: 1/1000 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | 1X PBS buffer with |
| 抗菌剂 | |
| 浓度 | 2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RARA |
| 全名 | retinoic acid receptor, alpha |
| 背景介绍 | This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010] |
| 生物功能 | Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (By similarity). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis. [UniProt] |
| 研究领域 | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 51 kDa |
| 翻译后修饰 | Phosphorylated on serine and threonine residues. Phosphorylation does not change during cell cycle. Phosphorylation on Ser-77 is crucial for transcriptional activity (By similarity). Phosphorylation by AKT1 is required for the repressor activity but has no effect on DNA binding, protein stability nor subcellular localization. Phosphorylated by PKA in vitro. This phosphorylation on Ser-219 and Ser-369 is critical for ligand binding, nuclear localization and transcriptional activity in response to FSH signaling. Sumoylated with SUMO2, mainly on Lys-399 which is also required for SENP6 binding. On all-trans retinoic acid (ATRA) binding, a confromational change may occur that allows sumoylation on two additional site, Lys-166 and Lys-171. Probably desumoylated by SENP6. Sumoylation levels determine nuclear localization and regulate ATRA-mediated transcriptional activity. Trimethylation enhances heterodimerization with RXRA and positively modulates the transcriptional activation. Ubiquitinated. |
检测图片 (1)
ARG62618 anti-Retinoic Acid Receptor alpha antibody [763] WB image
Western blot: Rat hippocampal lysate showing specific immunolabeling of the ~48 kDa RAR-alpha protein stained with ARG62618 anti-Retinoic Acid Receptor alpha antibody [763].
参考文献
克隆号文献
The effect pathway of retinoic acid through regulation of retinoic acid receptor alpha in gastric cancer cells.
Expression of retinoic acid receptor gamma correlates with retinoic acid sensitivity and metabolism in head and neck squamous cell carcinoma cell lines.
Retinoic acid receptor-alpha messenger RNA expression is increased and retinoic acid receptor-gamma expression is decreased in Barrett's intestinal metaplasia, dysplasia, adenocarcinoma sequence.
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