anti-RNA polymerase II antibody [8WG16]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [8WG16] recognizes RNA polymerase II
  • 反应物种: All
  • 应用: WB
  • 特异性: This antibody reacts with the highlyconserved heptapeptide repeat of thelargest subunit of eukaryotic RNApolymerase II. It inhibits promoterdirectedtranscription, but it does notinhibit elongation in the nonspecifictranscription assay.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 8WG16
  • 同位型: IgG2a
  • 靶点名称: RNA polymerase II
  • Brand:
CAT.NO. : ARG54635
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Product Details
概述
产品描述Mouse Monoclonal antibody [8WG16] recognizes RNA polymerase II
反应物种All
应用WB
特异性This antibody reacts with the highlyconserved heptapeptide repeat of thelargest subunit of eukaryotic RNApolymerase II. It inhibits promoterdirectedtranscription, but it does notinhibit elongation in the nonspecifictranscription assay.
宿主Mouse
克隆Monoclonal
克隆号8WG16
同位型IgG2a
靶点名称RNA polymerase II
抗原物种Wheat
抗原Purified wheat germ RNApolymerase II.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Protein G affinity chromatography
缓冲液PBS (pH 7.4)
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
基因名称rpoB
全名RNA polymerase beta chain
生物功能DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. [UniProt]
研究领域Gene Regulation antibody; Microbiology and Infectious Disease antibody
预测分子量122 kDa
参考文献

克隆号文献

Aberrant transcriptional regulations in cancers: genome, transcriptome and epigenome analysis of lung adenocarcinoma cell lines.

ChIP-seq / Human

Suzuki A et al.
Nucleic Acids Res.,  (2014)

publication_link

 

hr_line

Human cytomegalovirus pUL79 is an elongation factor of RNA polymerase II for viral gene transcription.

Perng YC et al.
PLoS Pathog.,  (2014)

publication_link

 

hr_line

Hierarchical molecular events driven by oocyte-specific factors lead to rapid and extensive reprogramming.

WB / 

Jullien J et al.
Mol Cell.,  (2014)

publication_link

 

hr_line

Construction of mate pair full-length cDNAs libraries and characterization of transcriptional start sites and termination sites.

ChIP-seq / Human

Matsumoto K et al.
Nucleic Acids Res.,  (2014)

publication_link

 

hr_line

Differential acetylation of histone H3 at the regulatory region of OsDREB1b promoter facilitates chromatin remodelling and transcription activation during cold stress.

ChIP / Rice

Roy D et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Rpb4 subunit functions mainly in mRNA synthesis by RNA polymerase II.

WB / 

Schulz D et al.
J Biol Chem.,  (2014)

publication_link

 

hr_line

Antisense-mediated FLC transcriptional repression requires the P-TEFb transcription elongation factor.

WB / Arabidopsis thaliana

Wang ZW et al.
Proc Natl Acad Sci U S A.,  (2014)

publication_link

 

hr_line

A comparison of the rest complex binding patterns in embryonic stem cells and epiblast stem cells.

ChIP-seq / Mouse

Seki M et al.
PLoS One.,  (2014)

publication_link

 

hr_line

The highly expressed methionine synthase gene of Neurospora crassa is positively regulated by its proximal heterochromatic region.

ChIP / Neurospora crassa

Yang S et al.
Nucleic Acids Res.,  (2014)

publication_link

 

hr_line

Nonproteolytic roles of 19S ATPases in transcription of CIITApIV genes.

Maganti N et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Sense transgene-induced post-transcriptional gene silencing in tobacco compromises the splicing of endogenous counterpart genes.

ChIP / 

Shin MR et al.
PLoS One.,  (2014)

publication_link

 

hr_line

The TAF9 C-terminal conserved region domain is required for SAGA and TFIID promoter occupancy to promote transcriptional activation.

Saint M et al.
Mol Cell Biol.,  (2014)

publication_link

 

hr_line

Separation of a functional deubiquitylating module from the SAGA complex by the proteasome regulatory particle.

IP / Saccharomyces cerevisiae

Lim S et al.
Nat Commun.,  (2013)

publication_link

 

hr_line

Mediator directs co-transcriptional heterochromatin assembly by RNA interference-dependent and -independent pathways.

ChIP / Schizosaccharomyces pombe

Oya E et al.
PLoS Genet.,  (2013)

publication_link

 

hr_line

Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae.

WB / Saccharomyces cerevisiae

Golla U et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Arabidopsis DEAD-box RNA helicase UAP56 interacts with both RNA and DNA as well as with mRNA export factors.

ICC/IF / Arabidopsis thaliana

Kammel C et al.
PLoS One.,  (2013)

publication_link

 

hr_line

The role of the Arabidopsis Exosome in siRNA-independent silencing of heterochromatic loci.

ChIP / Arabidopsis thaliana

Shin JH et al.
PLoS Genet.,  (2013)

publication_link

 

hr_line

CCN3/NOV gene expression in human prostate cancer is directly suppressed by the androgen receptor.

WB / Mouse

Wu L et al.
Oncogene.,  (2014)

publication_link

 

hr_line

Mutations in the Arabidopsis H3K4me2/3 demethylase JMJ14 suppress posttranscriptional gene silencing by decreasing transgene transcription.

Le Masson I et al.
Plant Cell.,  (2012)

publication_link

 

hr_line

Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS.

ChIP / Human

Tao Y et al.
Nucleic Acids Res.,  (2011)

publication_link

 

hr_line

Domains of Tra1 important for activator recruitment and transcription coactivator functions of SAGA and NuA4 complexes.

ChIP / Saccharomyces cerevisiae

Knutson BA et al.
Mol Cell Biol.,  (2011)

publication_link

 

hr_line

The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny.

ChIP / Caenorhabditis elegans

Rechtsteiner A et al.
PLoS Genet.,  (2010)

publication_link

 

hr_line

Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.

WB / Mouse

Tao Y et al.
PLoS One.,  (2010)

publication_link

 

hr_line

H2A.Z-containing nucleosomes mediate the thermosensory response in Arabidopsis.

ChIP / Arabidopsis thaliana

Kumar SV et al.
Cell.,  (2010)

publication_link

 

hr_line

RNA Pol II accumulates at promoters of growth genes during developmental arrest.

WB, ChIP-seq / Caenorhabditis elegans

Baugh LR et al.
Science.,  (2009)

publication_link

 

hr_line

PolV(PolIVb) function in RNA-directed DNA methylation requires the conserved active site and an additional plant-specific subunit.

WB / Arabidopsis thaliana

Lahmy S et al.
Proc Natl Acad Sci U S A.,  (2009)

publication_link

 

hr_line

A positive regulatory role for the mSin3A-HDAC complex in pluripotency through Nanog and Sox2.

ChIP / Mouse

Baltus GA et al.
J Biol Chem.,  (2009)

publication_link

 

hr_line
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