anti-RPE65 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
CAT.NO. : ARG41875
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes RPE65 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RPE65 |
| 抗原物种 | Human |
| 抗原 | Recombinant fusion protein corresponding to aa. 274-533 of Human RPE65. (NP_000320.1) |
| 偶联标记 | Un-conjugated |
| 別名 | Retinal pigment epithelium-specific 65 kDa protein; rd12; EC 3.1.1.64; LCA2; Retinol isomerase; sRPE65; RP20; BCO3; All-trans-retinyl-palmitate hydrolase; mRPE65; Retinoid isomerohydrolase |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | NCI-H460 | ||||||
| 实际分子量 | ~ 63 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | RPE65 |
| 全名 | retinal pigment epithelium-specific protein 65kDa |
| 背景介绍 | This gene encodes a protein which is located in the retinal pigment epithelium and is involved in the production of 11-cis retinal and in visual pigment regeneration. There are two forms of this protein, a soluble form called sRPE65, and a palmitoylated, membrane-bound form known as mRPE65. mRPE65 serves as the palmitoyl donor for lecithin retinol acyl transferase (LRAT), the enzyme that catalyzes the vitamin A to all trans retinol step of the chromophore regeneration process. Both mRPE65 and sRPE65 also serve as regulatory proteins, with the ratio and concentrations of these molecules playing a role in the inhibition of 11-cis retinal synthesis. Mutations in this gene have been associated with Leber congenital amaurosis type 2 (LCA2) and retinitis pigmentosa. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane; Lipid-anchor. Microsome membrane. Note=Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated. Undergoes light-dependent intracellular transport to become more concentrated in the central region of the retina pigment epithelium cells. [UniProt] |
| 预测分子量 | 61 kDa |
| 翻译后修饰 | Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A). [UniProt] |
检测图片 (3)
ARG41875 anti-RPE65 antibody IHC-Fr image
Immunohistochemistry: Mouse retinastained with ARG41875 anti-RPE65 antibody atdilution.
From Da Sun et al. Mol Ther Nucleic Acids. (2022), doi: 10.1016/j.omtn.2022.08.026, Fig. 3C.
ARG41875 anti-RPE65 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse eye tissue stained with ARG41875 anti-RPE65 antibody at 1:100 dilution.
ARG41875 anti-RPE65 antibody WB image
Western blot: 25 µg of NCI-H460 cell lysate stained with ARG41875 anti-RPE65 antibody at 1:1000 dilution.
参考文献
Effective gene therapy of Stargardt disease with PEG-ECO/ pGRK1-ABCA4-S/MAR nanoparticles
IHC-Fr / Mouse
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