anti-RPS10 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes RPS10
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, IHC-P, IP, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: RPS10
  • 抗原物种: Human
  • 抗原: Recombinant protein of Human RPS10
  • Brand:
CAT.NO. : ARG56314
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes RPS10
反应物种Hu, Ms, Rat
应用ICC/IF, IHC-P, IP, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称RPS10
抗原物种Human
抗原Recombinant protein of Human RPS10
偶联标记Un-conjugated
別名S10; DBA9; 40S ribosomal protein S10
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Mouse spleen and HT-29
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 6204 Human RPS10

GeneID: 67097 Mouse RPS10

GeneID: 81773 Rat RPS10

基因名称RPS10
全名ribosomal protein S10
背景介绍Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternate splicing results in multiple transcript variants that encode the same protein. Naturally occurring read-through transcription occurs between this locus and the neighboring locus NUDT3 (nudix (nucleoside diphosphate linked moiety X)-type motif 3).[provided by RefSeq, Feb 2011]
生物功能Component of the 40S ribosomal subunit. [UniProt]
预测分子量19 kDa
翻译后修饰Methylated by PRMT5. Methylation is necessary for its interaction with NPS1, its localization in the granular component (GC) region of the nucleolus, for the proper assembly of ribosomes, protein synthesis and optimal cell proliferation.
Monoubiquitinated by ZNF598 when a ribosome has stalled during translation of poly(A) sequences, leading to preclude synthesis of a long poly-lysine tail and initiate the ribosome quality control (RQC) pathway to degrade the potentially detrimental aberrant nascent polypeptide (PubMed:28065601, PubMed:28132843).
检测图片 (3)
  • ARG56314 anti-RPS10 antibody ICC/IF image

    Immunofluorescence: MCF7 cells stained with ARG56314 anti-RPS10 antibody.

  • ARG56314 anti-RPS10 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain stained with ARG56314 anti-RPS10 antibody at 1:100 dilution.

  • ARG56314 anti-RPS10 antibody WB image

    Western blot: Mouse spleen and HT-29 cell lysates stained with ARG56314 anti-RPS10 antibody.

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