anti-RRBP1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
- 抗原:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes RRBP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | RRBP1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide corresponding to a sequence of Human RRBP1. (EKEKKLTSDLGRAATRLQELLKTTQEQLAREKDTVKKLQEQLEKAED) |
| 偶联标记 | Un-conjugated |
| 別名 | Ribosome-binding protein 1; ES130; Ribosome receptor protein; hES; ES/130-related protein; ES/130; 180 kDa ribosome receptor homolog; RRp |
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| 实际分子量 | ~ 190 kDa |
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| 抗菌剂 | 0.05% Sodium azide |
| 稳定剂 | 4% Trehalose |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | RRBP1 |
| 全名 | ribosome binding protein 1 |
| 背景介绍 | This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012] |
| 生物功能 | Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Single-pass type III membrane protein. [UniProt] |
| 预测分子量 | 152 kDa |
ARG42962 anti-RRBP1 antibody ICC/IF image
Immunofluorescence: U2OS cells were blocked with 10% goat serum and then stained with ARG42962 anti-RRBP1 antibody (green) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
ARG42962 anti-RRBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42962 anti-RRBP1 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG42962 anti-RRBP1 antibody WB image
Western blot: 50 µg of sample under reducing conditions. Caco-2 and HepG2 whole cell lysates stained with ARG42962 anti-RRBP1 antibody at 0.5 µg/ml dilution, overnight at 4°C.
ARG42962 anti-RRBP1 antibody FACS image
Flow Cytometry: A549 cells were blocked with 10% normal goat serum and then stained with ARG42962 anti-RRBP1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
ARG42962 anti-RRBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42962 anti-RRBP1 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG42962 anti-RRBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42962 anti-RRBP1 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG42962 anti-RRBP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG42962 anti-RRBP1 antibody at 1 µg/ml dilution, overnight at 4°C.
ARG42962 anti-RRBP1 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal goat serum and then stained with ARG42962 anti-RRBP1 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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