anti-S100A4 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes S100A4
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Dog
  • 应用: IHC-P, WB
  • 特异性: Both variants (NP_002952.1; NP_062427.1) represent identical protein.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: S100A4
  • Brand:
CAT.NO. : ARG64101
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes S100A4
反应物种Hu
预测物种Ms, Rat, Dog
应用IHC-P, WB
特异性Both variants (NP_002952.1; NP_062427.1) represent identical protein.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称S100A4
抗原物种Human
抗原CNEFFEGFPDKQP
偶联标记Un-conjugated
別名S100 calcium-binding protein A4; Placental calcium-binding protein; 42A; MTS1; FSP1; CAPL; Metastasin; Protein S100-A4; P9KA; 18A2; Protein Mts1; Calvasculin; PEL98
应用说明
应用建议
应用推荐稀释比
IHC-P1 - 2 µg/ml
WB0.05 - 0.2 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 6275 Human S100A4

Swiss-port # P26447 Human Protein S100-A4

背景介绍The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
产品亮点Related products:
S100A4 antibodies; S100A4 Duos / Panels; Anti-Goat IgG secondary antibodies;
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预测分子量12 kDa
检测图片 (4)
  • ARG64101 anti-S100A4 / CAPL antibody WB image

    Western Blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG64101 anti-S100A4 / CAPL antibody at 0.03 µg/ml dilution.

  • ARG64101 anti-S100A4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64101 anti-S100A4 antibody at 2 µg/ml dilution followed by HRP-staining.

  • ARG64101 anti-S100A4 / CAPL antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Small Intestine. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG64101 anti-S100A4 / CAPL antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG64101 anti-S100A4 antibody WB image

    Western blot: 35 µg of peripheral blood mononuclear cell lysates (in RIPA buffer) stained with ARG64101 anti-S100A4 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.

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