anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Rabbit Polyclonal antibody recognizes SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) |
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| 反应物种 | Hu, Ms, Rat |
| 应用 | ICC/IF, WB |
| 特异性 | The antibody detects endogenous levels of dually phosphorylated JNK1 / JNK2 at Thr183 and Tyr185, and JNK3 at Thr221 and Tyr223. |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SAPK / JNK |
| 抗原物种 | Human |
| 抗原 | Peptide sequence around phosphorylation site of Thr183/Tyr185 (M-M-T(p)-P-Y(p)- V - V) derived from Human SAPK / JNK. |
| 偶联标记 | Un-conjugated |
| 別名 | JNK3A; Mitogen-activated protein kinase 10; p54bSAPK; Stress-activated protein kinase 1b; JNK3; c-Jun N-terminal kinase 3; Stress-activated protein kinase JNK3; EC 2.7.11.24; SAPK1b; p493F12; MAP kinase 10; PRKM10; MAPK 10; MAP kinase p49 3F12 |
| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
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| 纯化 | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| 缓冲液 | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | MAPK10 |
| 全名 | mitogen-activated protein kinase 10 |
| 背景介绍 | Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity |
| 生物功能 | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock (PubMed:22441692). [UniProt] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30205 NFkB Activation Antibody Panel ARG30294 Phospho SAPK / JNK Antibody Duo (Total, pT183/Y185) Related products: JNK antibodies; JNK ELISA Kits; JNK Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Treatment of Obesity with Celastrol Tumor microenvironments are shown to affect progression of several cancer subtypes Immune signaling protein TLR4 has opposing roles in breast cancer development Understanding Your Cells: Choose the right markers Related poster download: Toll-like Receptor.pdf |
| 研究领域 | Cancer antibody; Immune System antibody; Signaling Transduction antibody; NF-kB Activation Study antibody |
| 预测分子量 | 53 kDa |
| 翻译后修饰 | Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. Palmitoylation regulates subcellular location and axonal development. |
ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody WB image
Western blot: 30 µg of HeLa cells untreated or treated with TNF-alpha at 10, 20, or 30 min. The blots were stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody at 1:500 dilution.
ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody WB image
Western blot: Extracts from C6 cells untreated or treated with anisomycin. The blots were stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody.
ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody ICC/IF image
Immunofluorescence: Methanol-fixed HeLa cells stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody.
ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody WB image
Western blot: Extracts from 293 cells, treated with Anisomycin or calf intestinal phosphatase (CIP). The blots were stained with ARG51807 anti-SAPK / JNK phospho (Thr183 (221) / Tyr185 (223)) antibody.
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