anti-SARS-CoV-2 Spike protein (RBD) antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes SARS-CoV-2 Spike protein (RBD)
  • 反应物种: Virus
  • 应用: ELISA, ICC/IF, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: SARS-CoV-2 Spike protein (RBD)
  • 抗原物种: Virus
  • 抗原: A 19-amino acid synthetic peptide within the last 50 amino acids of SARS-CoV-2 Spike protein (RBD).
  • Brand:
CAT.NO. : ARG43345
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes SARS-CoV-2 Spike protein (RBD)
反应物种Virus
应用ELISA, ICC/IF, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称SARS-CoV-2 Spike protein (RBD)
抗原物种Virus
抗原A 19-amino acid synthetic peptide within the last 50 amino acids of SARS-CoV-2 Spike protein (RBD).
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
ICC/IF20 µg/ml
IHC-P0.5 µg/ml
WB2 - 5 µg/ml
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS and 0.02% Sodium azide.
抗菌剂0.02% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
检测图片 (4)
  • ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody ICC/IF image

    Immunofluorescence: 4% Paraformaldehyde-fixed Spike transfected 293 cells stained with ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody (green) at 20 µg/ml dilution. DAPI (blue) for nuclear staining.

  • ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody IHC-P image

    Immunohistochemistry: Formaldehyde-fixed and paraffin-embedded COVID-19 patient lung tissue. Tissue was blocked with 10% serum for 1 hour at RT. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0). Samples were stained with ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody at 0.5 µg/ml dilution, overnight at 4°C. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Spike RBD protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

  • ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody WB image

    Western blot: 30 ng of SARS-CoV-2 Spike RBD recombinant protein stained with ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody at 1 µg/ml (left) or 2 µg/ml (right) dilution, for 1 hour incubation at RT, in 5% NFDM/TBST.

  • ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody ELISA image

    Direct ELISA: SARS-CoV-2 Spike RBD recombinant protein as coating antigen and ARG43345 anti-SARS-CoV-2 Spike protein (RBD) antibody as the primary antibody.

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