anti-SCGN / Secretagogin antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes SCGN / Secretagogin
  • 反应物种: Hu, Ms, Rat
  • 应用: ICC/IF, IHC-Fr, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: SCGN / Secretagogin
  • 抗原物种: Human
  • 抗原: Full-length recombinant Human secretagogin protein.
  • Brand:
CAT.NO. : ARG10739
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes SCGN / Secretagogin
反应物种Hu, Ms, Rat
应用ICC/IF, IHC-Fr, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称SCGN / Secretagogin
抗原物种Human
抗原Full-length recombinant Human secretagogin protein.
偶联标记Un-conjugated
別名SEGN; DJ501N12.8; setagin; SECRET; CALBL; Secretagogin
应用说明
应用建议
应用推荐稀释比
ICC/IF1:1000 - 1:2000
IHC-Fr1:1000 - 1:2000
WB1:1000 - 1:5000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification.
缓冲液PBS and 50% Glycerol.
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 10590 Human SCGN

GeneID: 214189 Mouse SCGN

GeneID: 306942 Rat SCGN

基因名称SCGN
全名secretagogin, EF-hand calcium binding protein
背景介绍The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq, Jul 2008]
预测分子量32 kDa
检测图片 (4)
  • ARG10739 anti-SCGN / Secretagogin antibody IHC image

    IHC: Adult Mouse olfactory bulb floating section stained with ARG10739 anti-SCGN / Secretagogin antibody at 1:1000 (red) and co-stained with our chicken polyclonal antibody to calretinin at 1:1000 (green). Secretagogin is predominantly expressed in cells in external plexiform lay (EPL) and granular cell lay (GCL) in olfactory bulb. Secretagogin coexists with calretinin in some cells in GCL, so these cells appear yellow.  Secretagogin is found in both cell body and processes. Blue is a DNA stain.

  • ARG10739 anti-SCGN / Secretagogin antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Rat brain cerebellum stained with ARG10739 anti-SCGN / Secretagogin antibody (green) at 1:1000 dilution and costained with Mouse mAb to calbindin (red) at 1:1000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)

    The SCGN antibody stains granule cells and interneurons while calbindin antibody reveals protein expressed in dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum.

  • ARG10739 anti-SCGN / Secretagogin antibody WB image

    Western blot: Rat pancreas lysate stained with ARG10739 anti-SCGN / Secretagogin antibody at 1:1000 dilution.

  • ARG10739 anti-SCGN / Secretagogin antibody WB image

    Western blot: Rat pancreas, Rat olfactory bulb and Mouse olfactory bulb lysates stained with ARG10739 anti-SCGN / Secretagogin antibody (green) at 1:1000 dilution.

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