anti-Septin 3 antibody
产品描述 | Goat Polyclonal antibody recognizes Septin 3 |
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反应物种 | Hu |
预测物种 | Ms, Rat, Cow, Dog, Pig |
应用 | IHC-P, WB |
特异性 | This antibody is expected to recognize both reported isoforms according to NP_663786.1 and NP_061979.3. |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | Septin 3 |
抗原物种 | Human |
抗原 | SELVPEPRPKPA-C |
偶联标记 | Un-conjugated |
別名 | SEP3; Neuronal-specific septin-3; bK250D10.3 |
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
数据库连接 | |
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背景介绍 | This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
研究领域 | Cell Biology and Cellular Response antibody |
预测分子量 | 41 kDa |
翻译后修饰 | Phosphorylated by PKG on serine residues. Phosphorylated by PKG on Ser-91 (By similarity). |
ARG63204 anti-Septin 3 antibody WB image
Western Blot: Human Brain lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63204 anti-Septin 3 antibody at 1 µg/ml dilution.
ARG63204 anti-Septin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cortex tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63204 anti-Septin 3 antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG63204 anti-Septin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human testis tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63204 anti-Septin 3 antibody at 3.75 µg/ml dilution followed by AP-staining.
ARG63204 anti-Septin 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63204 anti-Septin 3 antibody at 3.75 µg/ml dilution followed by AP-staining.
