Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-1 Protein Mouse Monoclonal Antibody (3B5)

Key features and details

  • 产品: 抗血清4禽腺病毒(FAdV-4 )Fiber-1蛋白特异性鼠单克隆抗体 (3B5)
  • Clone: 3B5
  • Host: Mouse
  • Species Reactivity: FAdV-C (FAdV-4/10) Fiber-1蛋白
  • Application: IFA,IP
  • Immunogen : FAdV-4 Fiber-1基因的原核表达产物GST-Fiber-1
  • Clone Type: Monoclonal
  • Storage: -20.0°C
  • Brand:
CAT.NO. : AXA0024
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Product Details
概述

宿主

小鼠

抗体亚型

IgG

克隆性

单克隆

用途

IFA、IP

反应性

FAdV-C (FAdV-4/10) Fiber-1 蛋白

应用

应用

推荐稀释比例

IFA

1:1000

IP

1:1000


*最佳稀释度/浓度应由研究人员确定
产品信息

性状

液体

储存缓冲液

PBS,20% 甘油;防腐剂:0.05% 叠氮化钠

储存条件

-20°C 保存, 1 年有效,避免反复冻融; 4℃环境中可储存 1-2 周。

浓度

1 mg/mL

免疫原

FAdV-4 Fiber-1 基因的原核表达产物 GST-Fiber-1


注意事项:
仅供实验室研究使用,不可用于人类或动物的任何临床、治疗或诊断用途,不供动物或人类食用。
购买者不得且同意不允许第三方分析、复制、逆向工程或以其他方式尝试确定产品的结构或序列。
相关数据

A:感染了 FAdV-4 的 LMH 细胞,B:感染了 FAdV-8b 的 LMH 细胞
C:转染了 pcDNA3.1-Fiber-1 的 LMH 细胞,D:转染了 pcDNA3.1 的LMH 细胞
IFA 结果表明,单抗 3B5 可有效识别感染了 FAdV-4 的 LMH 细胞和过表达 FAdV-4 Fiber-1 的 LMH 细胞


IP:3B5 mAb
WB:抗 Fiber-1 鼠源

A:转染了 pcDNA3.1-Fiber-1 的 LMH 细胞,B:转染了 pcDNA3.1 的LMH 细胞对照,C:感染了FAdV-4-WT 的 LMH 细胞,D:未感染的 LMH 细胞对照
IP 结果表明,单抗 3B5 可有效免疫沉淀 FAdV-4 Fiber-1 蛋白
Reference
1. Shao H, Lu Y, Wang W, et al. Two novel monoclonal antibodies against fiber-1 protein of FAdV-4 and their application in detection of FAdV-4/10. BMC Vet Res. 2019;15(1):232. doi:10.1186/s12917-019-1987-5
2. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
3. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. doi:10.1186/s13567-021-00907-z
4. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
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