Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein Mouse Monoclonal Antibody (1C9)
Key features and details
- Product:
- Clone:
- Host:
- Species Reactivity:
- Application:
- Clone Type:
- Storage:
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Brand:
CAT.NO. : AXA0025
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Product Details
Background
Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein is a structural glycoprotein located on the viral capsid surface alongside Fiber-1 it plays a complementary role in viral infection by mediating interactions with host cell receptors that may differ from those targeted by Fiber-1 it possesses unique immunogenic epitopes contributing to the overall immune response against FAdV-4 infection and exhibits distinct structural and functional characteristics compared to Fiber-1 of the same serotype as a specific viral component it serves as a valuable target for the development of serological diagnostic tools and subunit vaccines enhancing the specificity and efficacy of FAdV-4 detection and prevention it also provides insights into the viral tropism and pathogenic mechanisms of FAdV-4 through studies on its binding specificity and biological functions.
Application
|
Application |
Recommended Dilution Ratio |
|
IFA |
1:500-1:1000 |
|
WB |
1:2000-1:5000 |
|
IP |
1:200-1:1000 |
*The optimal dilution ratio/concentration should be determined by researchers.
Overview
|
Host |
Mouse |
|
Antibody Subtype |
IgG |
|
Clonality |
Monoclonal |
|
Applications |
IFA, WB, IP |
|
Reactivity |
FAdV-C (FAdV-4/10) Fiber-2 Protein |
|
Appearance |
Liquid |
|
Storage Buffer |
PBS, 20% Glycerol; Preservative: 0.05% Sodium Azide |
|
Storage Conditions |
Store at -20°C, valid for 1 year. Avoid repeated freeze-thaw cycles; can be stored at 4°C for 1-2 weeks. |
|
Concentration |
1 mg/mL |
|
Immunogen |
GST-Fiber-2, the Prokaryotic Expression Product of FAdV-4 Fiber-2 Gene |
Data
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-5, C: LMH cells infected with FAdV-6, D: LMH cells infected with FAdV-7a, E: LMH cells infected with FAdV-8a, F: LMH cells infected with FAdV-8b, G: LMH cells infected with FAdV-9, H: LMH cells infected with FAdV-10, I:Uninfected LMH cells
IFA results demonstrate that monoclonal antibodies 1C9 and 3C2 can effectively recognize LMH cells infected with FAdV-4 and FAdV-10, monoclonal antibody 1D7 can efficiently identify LMH cells infected with FAdV-4, and all three monoclonal antibodies exhibit good specificity.
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
WB results demonstrate that monoclonal antibody 1C9 can effectively recognize the Fiber-2 proteins of FAdV-4 and FAdV-10.
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
IP results demonstrate that monoclonal antibody 1D7 can effectively immunoprecipitate the Fiber-2 protein of FAdV-4.
A: LMH cells infected with FAdV-4, B: LMH cells infected with FAdV-10, C: Uninfected LMH cells
IP results demonstrate that monoclonal antibody 3C2 can effectively immunoprecipitate the Fiber-2 proteins of FAdV-4 and FAdV-10.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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