Anti-Serotype 4 Fowl Adenovirus (FAdV-4) Fiber-2 Protein Mouse Monoclonal Antibody (3C2)
Key features and details
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- Clone:
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- Species Reactivity:
- Application:
- Immunogen :
- Clone Type:
- Storage:
-
Brand:
CAT.NO. : AXA0026
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Product Details
概述
|
宿主 |
小鼠 |
|
抗体亚型 |
IgG |
|
克隆性 |
单克隆 |
|
用途 |
IFA、IP |
|
反应性 |
FAdV-C (FAdV-4/10) Fiber-2 蛋白 |
应用
|
应用 |
推荐稀释比例 |
|
IFA |
1:1000 |
|
WB |
1:2000-1:5000 |
|
IP |
1:1000 |
*最佳稀释度/浓度应由研究人员确定
产品信息
|
性状 |
液体 |
|
储存缓冲液 |
PBS，20% 甘油；防腐剂：0.05% 叠氮化钠 |
|
储存条件 |
-20°C 保存， 1 年有效，避免反复冻融; 4℃环境中可储存 1-2 周。 |
|
浓度 |
1 mg/mL |
|
免疫原 |
FAdV-4 Fiber-2 基因的原核表达产物 GST-Fiber-2 |
注意事项：
仅供实验室研究使用，不可用于人类或动物的任何临床、治疗或诊断用途，不供动物或人类食用。
购买者不得且同意不允许第三方分析、复制、逆向工程或以其他方式尝试确定产品的结构或序列。
相关数据
A：感染了 FAdV-4 的 LMH 细胞，B：感染了 FAdV-5 的 LMH 细胞，C：感染了 FAdV-6 的 LMH 细胞，
D：感染了 FAdV-7a 的 LMH 细胞，E：感染了 FAdV-8a 的 LMH 细胞，F：感染了 FAdV-8b 的 LMH 细胞，
G: 感染了 FAdV-9 的 LMH 细胞，H：感染了 FAdV-10 的 LMH 细胞，I：未感染的 LMH 细胞
IFA 结果表明，单抗 1C9，3C2 可有效识别感染了 FAdV-4 与 FAdV-10 的 LMH 细胞；
单抗 1D7 可有效识别感染了 FAdV-4 的 LMH 细胞；且三株单抗具有良好的特异性
A：感染了 FAdV-4 的 LMH 细胞，B：感染了 FAdV-10 的 LMH 细胞，C：未感染的 LMH 细胞
WB 结果表明，单抗 1C9 可有效识别 FAdV-4 和 FAdV-10 的 Fiber-2 蛋白
A：感染了 FAdV-4 的 LMH 细胞，B：感染了 FAdV-10 的 LMH 细胞，C：未感染的 LMH 细胞
IP 结果表明，单抗 1D7 可有效免疫沉淀 FAdV-4 的 Fiber-2 蛋白
A：感染了 FAdV-4 的 LMH 细胞，B：感染了 FAdV-10 的 LMH 细胞，C：未感染的 LMH 细胞
IP 结果表明，单抗 3C2 可有效免疫沉淀 FAdV-4 和 FAdV-10 的 Fiber-2 蛋白
Reference
1. Wang P, Zhang J, Wang W, et al. A novel monoclonal antibody efficiently blocks the infection of serotype 4 fowl adenovirus by targeting fiber-2. Vet Res. 2018;49(1):29. doi:10.1186/s13567-018-0525-y
2. Xie S, Zhang J, Chen H, et al. Development of colloidal gold-based test strip for rapid detection of serotype 4 fowl adenovirus. J Virol Methods. 2021;296:114231. doi:10.1016/j.jviromet.2021.114231
3. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
4. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. Published 2021 Feb 27. doi:10.1186/s13567-021-00907-z
5. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
6. Mu Y, Xie Q, Wang W, et al. A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge. Front Vet Sci. 2021;8:759418. doi:10.3389/fvets.2021.759418
7. Wang W, Liu Q, Li T, et al. Fiber-1, Not Fiber-2, Directly Mediates the Infection of the Pathogenic Serotype 4 Fowl Adenovirus via Its Shaft and Knob Domains. J Virol. 2020;94(17):e00954-20. doi:10.1128/JVI.00954-20
2. Xie S, Zhang J, Chen H, et al. Development of colloidal gold-based test strip for rapid detection of serotype 4 fowl adenovirus. J Virol Methods. 2021;296:114231. doi:10.1016/j.jviromet.2021.114231
3. Xie Q, Wang W, Kan Q, et al. FAdV-4 without Fiber-2 Is a Highly Attenuated and Protective Vaccine Candidate. Microbiol Spectr. 2022;10(1):e0143621. doi:10.1128/spectrum.01436-21
4. Xie Q, Cao S, Zhang W, et al. A novel fiber-2-edited live attenuated vaccine candidate against the highly pathogenic serotype 4 fowl adenovirus. Vet Res. 2021;52(1):35. Published 2021 Feb 27. doi:10.1186/s13567-021-00907-z
5. Xie Q, Wang W, Li L, et al. Domain in Fiber-2 interacted with KPNA3/4 significantly affects the replication and pathogenicity of the highly pathogenic FAdV-4. Virulence. 2021;12(1):754-765. doi:10.1080/21505594.2021.1888458
6. Mu Y, Xie Q, Wang W, et al. A Novel Fiber-1-Edited and Highly Attenuated Recombinant Serotype 4 Fowl Adenovirus Confers Efficient Protection Against Lethal Challenge. Front Vet Sci. 2021;8:759418. doi:10.3389/fvets.2021.759418
7. Wang W, Liu Q, Li T, et al. Fiber-1, Not Fiber-2, Directly Mediates the Infection of the Pathogenic Serotype 4 Fowl Adenovirus via Its Shaft and Knob Domains. J Virol. 2020;94(17):e00954-20. doi:10.1128/JVI.00954-20
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