Anti-Serotype 8b Fowl Adenovirus (FAdV-8b) Fiber Protein Mouse Monoclonal Antibody (5F10)
Key features and details
- Product:
- Clone:
- Host:
- Species Reactivity:
- Application:
- Immunogen :
- Clone Type:
- Storage:
-
Brand:
CAT.NO. : AXA0029
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Size:
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Product Details
Background
Serotype 8b Fowl Adenovirus (FAdV-8b) Fiber Protein is a key structural protein of FAdV-8b, a member of the Aviadenovirus genus. It is a protruding fiber-like structure on the viral capsid, playing a crucial role in viral host cell recognition and entry by binding to specific receptors on the surface of host cells. This protein exhibits distinct immunogenic properties, capable of inducing the host to produce specific antibodies, and serves as an important target for the development of FAdV-8b detection methods and subunit vaccines. Its amino acid sequence and functional domains have characteristics that distinguish it from other FAdV serotypes, which are closely related to its host tropism and pathogenicity.
Application
|
Application |
Recommended Dilution Ratio |
|
IFA |
1:500-1:1000 |
|
WB |
1:2000-1:5000 |
*The optimal dilution ratio/concentration should be determined by researchers.
Overview
|
Host |
Mouse |
|
Antibody Subtype |
IgG |
|
Clonality |
Monoclonal |
|
Applications |
IFA, WB |
|
Reactivity |
FAdV-7 Fiber Protein, FAdV-8b Fiber Protein |
|
Appearance |
Liquid |
|
Storage Buffer |
PBS, 20% Glycerol; Preservative: 0.05% Sodium Azide |
|
Storage Conditions |
Store at -20°C, valid for 1 year. Avoid repeated freeze-thaw cycles; can be stored at 4°C for 1-2 weeks. |
|
Concentration |
1 mg/ml |
|
Immunogen |
GST-Fiber, the Prokaryotic Expression Product of FAdV-8b Fiber Gene |
Data
WB and IFA results demonstrate that monoclonal antibodies 4D9 and 5F10 can effectively react with LMH cells overexpressing FAdV-8b Fiber and LMH cells infected with FAdV-8b.
Western Blot (WB) was used for specificity detection. The results demonstrate that monoclonal antibody 5F10 can effectively recognize FAdV-7 and FAdV-8b Fiber proteins, while monoclonal antibody 4D9 can only effectively recognize FAdV-8b Fiber protein.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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